Chen Chien-Wei, Wu Li-Ting, Chiu Po-Hao, Chen Yen-Po, Huang Wen-Ching
Department of Physical Education, Health, and Recreation, Teachers College, National Chiayi University, Chiayi 621302, Taiwan.
Department of Exercise and Health Science, College of Human Development and Health, National Taipei University of Nursing and Health Sciences, Taipei 112303, Taiwan.
Int J Med Sci. 2025 Aug 11;22(14):3722-3736. doi: 10.7150/ijms.115387. eCollection 2025.
Individuals often suffer from insufficient or disrupted sleep due to night shifts, work pressure, and irregular lifestyles. Sleep deprivation (SD), defined as an intentional or unintentional reduction in sleep quality or quantity, has been associated with an increased risk of metabolic disorders, gut dysbiosis, emotional disturbances, and diminished performance in occupational and physical activities. Functional probiotics have been shown to regulate physiological homeostasis and ameliorate diseases through their impact on the microbiota and various physiological pathways. In this study, we employed the modified multiple platform method (MMPM) to induce SD in an animal model, simulating physiological dysregulation and psychological characteristics associated with SD. We further investigated whether exercise and the probiotic K6 could mitigate the effects of SD on physiological homeostasis, neuropsychological function, inflammation, circadian rhythm, and exercise capacity. We found that the probiotic K6 significantly alleviated depression and anxiety while improving glucose intolerance and declining endurance capacity in the SD model. Elevated injury indexes (CK and LDH) induced by SD combined with exercise training were also significantly reduced under K6 supplementation. In the liver and muscle, SD alone or combined with exercise led to inflammation () and dysregulated circadian gene expression (, ), both of which were mitigated by K6 supplementation. In the intestine, hypothalamus, and hippocampus, SD or SD combined with exercise-induced inflammation (, ) and tight junction hyperpermeability () were alleviated with K6 supplementation, as were the circadian genes (, ) and corticotropin-releasing hormone receptor genes (, ) in hypothalamus and hippocampus under SD alone or combined with exercise. The functional probiotic K6 improved physiological adaption, neuropsychological behaviors, and exercise performance with the implementation of SD and exercise training, potentially through regulation of inflammation, circadian rhythm, and stress response, contributing to overall health maintenance. The K6 probiotic strain may serve as a nutritional strategy to mitigate health risks and enhance performance affected by sleep deprivation.
由于夜班、工作压力和不规律的生活方式，人们常常睡眠不足或睡眠紊乱。睡眠剥夺（SD）被定义为睡眠质量或睡眠时间有意或无意的减少，它与代谢紊乱、肠道菌群失调、情绪障碍以及职业和体育活动表现下降的风险增加有关。功能性益生菌已被证明可通过影响微生物群和各种生理途径来调节生理稳态并改善疾病。在本研究中，我们采用改良多平台法（MMPM）在动物模型中诱导SD，模拟与SD相关的生理失调和心理特征。我们进一步研究了运动和益生菌K6是否可以减轻SD对生理稳态、神经心理功能、炎症、昼夜节律和运动能力的影响。我们发现，在SD模型中，益生菌K6显著减轻了抑郁和焦虑，同时改善了葡萄糖不耐受并降低了耐力。补充K6后，SD联合运动训练诱导的损伤指标（CK和LDH）也显著降低。在肝脏和肌肉中，单独的SD或SD与运动联合导致炎症（）和昼夜节律基因表达失调（，），补充K6均可减轻这些情况。在肠道、下丘脑和海马体中，补充K6可减轻SD或SD与运动联合诱导的炎症（，）和紧密连接高通透性（），单独的SD或SD与运动联合时，下丘脑和海马体中的昼夜节律基因（，）和促肾上腺皮质激素释放激素受体基因（，）也是如此。功能性益生菌K6通过实施SD和运动训练改善了生理适应性、神经心理行为和运动表现，可能是通过调节炎症、昼夜节律和应激反应，有助于维持整体健康。K6益生菌菌株可能作为一种营养策略，以减轻健康风险并提高受睡眠剥夺影响的表现。
