Sugiri D, Behrendt H, Seemayer N H
Zentralbl Bakteriol Mikrobiol Hyg B. 1985 Dec;181(3-5):226-39.
Macrophages of cell line IC-21 were exposed to extracts and fractions of two samples of city smog (CSE 16 and 17) from the heavy industrialized Rhine-Ruhr-area. Cytotoxic effects of extracts and fractions were analysed in various concentrations and periods of incubation. As cytotoxic parameters were determined frequencies of mitosis and pycnosis of nuclei as well as occurrence of multinucleated giant cells. An increasing dosage of noxae showed a reduction of mitotic rate, a rise of pycnosis of nuclei and of multinucleated giant cells. For both city smog extracts these effects depended on incubation period and concentration of noxae. While the global extract of city smog no. 16 was always more effective than its fractions, with city smog no. 17 the strongest alterations were demonstrable by its cyclohexane-fraction and partly by its methanol-fraction. Based on air volume of collection both samples of city smog revealed a comparable cytotoxic effect. In relation to benzo(a)pyrene-content, however, city smog no. 17 was considerable more cytotoxic than city smog no. 16. These results confirm again cytotoxicity of city smog. It can be assumed that both samples of city smog impair defense mechanisms of the lung.
将细胞系IC - 21的巨噬细胞暴露于来自重工业发达的莱茵 - 鲁尔地区的两份城市烟雾样本（CSE 16和17）的提取物和馏分中。分析了不同浓度和孵育时间下提取物和馏分的细胞毒性作用。作为细胞毒性参数，测定了有丝分裂频率、细胞核固缩以及多核巨细胞的出现情况。随着有害物质剂量的增加，有丝分裂率降低，细胞核固缩以及多核巨细胞增多。对于这两份城市烟雾提取物，这些效应取决于孵育时间和有害物质的浓度。虽然城市烟雾16号的整体提取物总是比其馏分更有效，但对于城市烟雾17号，环己烷馏分以及部分甲醇馏分表现出最强的变化。基于收集的空气量，两份城市烟雾样本显示出相当的细胞毒性作用。然而，就苯并(a)芘含量而言，城市烟雾17号的细胞毒性明显高于城市烟雾16号。这些结果再次证实了城市烟雾的细胞毒性。可以假定两份城市烟雾样本都会损害肺部的防御机制。
