Wang Yinghan, Pan Tingting, Hu Nan, Gao Guangtai, Jia Xiaorui, Zhang Yinghui, Song Chunru, Yin Chunying, Liu Yuling
Key Laboratory for Research and Development of Chinese Traditional Medicine of Hebei Province, Institute of Chinese Materia Medica, Chengde Medical College, Key Research Laboratory of Anti-Dementia of Traditional Chinese Medicine of Hebei Province, Chengde, Hebei, China.
Institute of Chinese Materia Medica, Chengde Medical College, Chengde, Hebei, China.
Gastroenterol Res Pract. 2025 Sep 9;2025:2125189. doi: 10.1155/grp/2125189. eCollection 2025.
Diarrhea-predominant irritable bowel syndrome (D-IBS) is a clinically common functional intestinal disease, classified into "diarrhea," "abdominal pain," and "depression syndrome" categories according to traditional Chinese medicine (TCM). The exact pathogenesis of D-IBS is still not fully understood. Gut microbiota regulates gastrointestinal nerve, endocrine, and immune functions and maintains gastrointestinal homeostasis through interaction with the brain-gut axis. In this study, we assessed the changes in gut microbiota in a D-IBS rat model with liver depression, spleen deficiency, and liver depression and spleen deficiency syndrome. We also discussed the biological basis of liver depression and spleen deficiency syndrome and the associations among the three syndromes from the perspective of gut microbiota. Ninety rats were divided into nine groups randomly: normal group (ZC), spleen deficiency syndrome groups (four PX groups), liver depression syndrome groups (two GY groups), and liver depression and spleen deficiency syndrome groups (two GYPX groups). The abdominal wall withdrawal reflex (AWR) test detected visceral sensitivity, while changes in gut microbiota were analyzed using 16S rRNA sequencing. The visceral sensitivity of rats in the model group was significantly higher than that in the ZC group, and the visceral sensitivity of the GYPX groups was significantly higher compared to the PX and GY groups. 16S rRNA sequencing analysis showed that the D-IBS model gut microbiota's species number, alpha diversity, and beta diversity were changed; the Bacteroidota increased, and the Firmicutes decreased in the model group. The abundance of pathogenic bacteria, such as Bacteroidales, significantly increased in the GYPX groups compared to other groups. Oral administration of senna combined with restraint stress had different effects on visceral hypersensitivity, gut microbiota composition, and metabolic pathways in rats with D-IBS liver depression and spleen deficiency syndrome, and the liver depression factors play an important role in the pathogenesis of liver depression and spleen deficiency syndrome in D-IBS.
腹泻型肠易激综合征（D-IBS）是一种临床常见的功能性肠道疾病，根据中医理论可分为“泄泻”“腹痛”“郁证”等证型。D-IBS的确切发病机制仍未完全明确。肠道微生物群通过与脑-肠轴相互作用调节胃肠神经、内分泌和免疫功能，维持胃肠内环境稳定。本研究评估了肝郁脾虚、肝郁脾虚证D-IBS大鼠模型肠道微生物群的变化。我们还从肠道微生物群的角度探讨了肝郁脾虚证的生物学基础以及三种证型之间的关联。将90只大鼠随机分为9组：正常组（ZC）、脾虚证组（4个PX组）、肝郁证组（2个GY组）、肝郁脾虚证组（2个GYPX组）。采用腹壁回撤反射（AWR）试验检测内脏敏感性，运用16S rRNA测序分析肠道微生物群的变化。模型组大鼠的内脏敏感性显著高于ZC组，GYPX组的内脏敏感性显著高于PX组和GY组。16S rRNA测序分析显示，D-IBS模型肠道微生物群的物种数量、α多样性和β多样性发生改变；模型组拟杆菌门增加，厚壁菌门减少。与其他组相比，GYPX组中拟杆菌目等病原菌的丰度显著增加。口服番泻叶联合束缚应激对D-IBS肝郁脾虚证大鼠的内脏高敏感性、肠道微生物群组成及代谢途径有不同影响，肝郁因素在D-IBS肝郁脾虚证的发病机制中起重要作用。
