Jayakumar Srilekha, Subramanian Anbarasu, Sabarinathan S, Shalini H, John Bindu Meera, Saravanan R
Department of Conservative Dentistry and Endodontics, Chettinad Dental College and Research Institute, Kelambakkam, Affiliated to The Tamil Nadu Dr. MGR Medical University, Chennai, Tamil Nadu, India.
Department of Dental Surgery, Government Kallakurichi Medical College and Hospital, Kallakurichi, Affiliated to The Tamil Nadu Dr. MGR Medical University, Chennai, Tamil Nadu, India.
J Conserv Dent Endod. 2025 Sep;28(9):875-880. doi: 10.4103/JCDE.JCDE_416_25. Epub 2025 Sep 5.
is a keystone organism associated with secondary endodontic infections. Numerous virulence traits are displayed by this bacterium, which coaggregates with other species and is speculated to function as a "supportive bridge" between primary and secondary invaders in the formation of root canal biofilms.
To evaluate the antimicrobial efficacy of (ZO) (Ginger) and (AS) (Garlic) against .
Extracts were prepared by powdering freshly dried ginger and garlic, and the extraction process was done using a Soxhlet apparatus. Broth microdilution assay and microtiter plate assay of the ginger and garlic extracts were done for evaluating antibacterial activity. Gas chromatography-mass spectrometry analysis was done to identify phytochemical constituents present in extracts responsible for antibacterial action. Molecular docking was done to evaluate the interaction between phytoconstituents and the target protein (NanA) of .
Statistical analysis was done by IBM SPSS version 30.0. One-way ANOVA test was used for intergroup comparison.
The minimum inhibitory concentration was determined to be 0.9 μg/mL for ZO and AS extracts. Both Ginger and Garlic extract showed a dose-dependent activity on growth, with the highest inhibition at 500 μg/mL. Docking showed N-amyl isovalerate in ginger and furaneol in garlic exhibited the strongest binding affinity with the target protein NanA.
Garlic and ginger extracts both showed better antibacterial activity against than 3% sodium hypochlorite, indicating that they could potentially utilized as a natural alternative irrigant.
是与继发性牙髓感染相关的关键微生物。这种细菌表现出许多毒力特征，它与其他物种共聚，据推测在根管生物膜形成过程中作为原发性和继发性入侵者之间的“支持桥梁”发挥作用。
评估姜（ZO）和大蒜（AS）对的抗菌效果。
将新鲜干燥的姜和大蒜磨成粉末制备提取物，提取过程使用索氏提取器进行。对姜和大蒜提取物进行肉汤微量稀释试验和微量滴定板试验以评估抗菌活性。进行气相色谱 - 质谱分析以鉴定提取物中负责抗菌作用的植物化学成分。进行分子对接以评估植物成分与的靶蛋白（NanA）之间的相互作用。
使用IBM SPSS 30.0版进行统计分析。单向方差分析用于组间比较。
ZO和AS提取物的最低抑菌浓度确定为0.9μg/mL。姜和大蒜提取物对的生长均表现出剂量依赖性活性，在500μg/mL时抑制作用最强。对接显示姜中的异戊酸戊酯和大蒜中的呋喃醇与靶蛋白NanA表现出最强的结合亲和力。
大蒜和姜提取物对的抗菌活性均优于3%次氯酸钠，表明它们有可能用作天然替代冲洗剂。
