Peremykina Anastasiya, Cheranev Valery, Shilyaev Alexey Y, Krivoy Andrey, Repinskaia Zhanna, Korostin Dmitriy, Rebrikov Denis
Department of Outpatient Clinical Research Development, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. Kulakov, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Medical University, Moscow, Russia.
Front Microbiol. 2025 Sep 2;16:1630092. doi: 10.3389/fmicb.2025.1630092. eCollection 2025.
The study of the cervicovaginal microbiome is a critical area of research in medical science. According to scientific data, microorganisms inhabiting the lower female genital tract may influence susceptibility to and persistence of human papillomavirus (HPV), as well as the development and progression of squamous intraepithelial lesions (SIL) toward cervical cancer (CC).
The study included 67 patients with a histological diagnosis of low-grade squamous intraepithelial lesion (LSIL) and 85 patients with high-grade squamous intraepithelial lesion (HSIL). The cervical canal microbiome of patients with LSIL ( = 31) and HSIL ( = 45) was examined using the 16S rRNA gene sequencing method, while the microbiocenosis of the remaining patients with LSIL ( = 36) and HSIL ( = 40) was analyzed using PCR-RT.
There are patients with HSIL on average older than patients with LSIL about 3 years. HPV 16 was found to be the most common virus type in patients with SIL. When determining of 16S rRNA genes of prokaryotic microorganisms in women of reproductive age with SIL by the new generation sequencing, it turned out that the cervical canal microbiome is inhabited by many atypical representatives (soil, aquatic and aerobacteria). In addition to the genera and others may be included in the microbial composition of the cervical canal. Dysbiotic disorders were more common in patients with HSIL. spp. always accompanied aerobic vaginitis (AV), whereas bacterial vaginosis (BV) was associated with the genera and spp. When comparing the two methods, the genera and may be included in the microbial biomass in a significant proportion. Eubacterium spp. did not occur in the 16S sequencing method. Some representatives are detected together with each other in the RT-qPCR method (Femoflor - 16), e.g., spp. + spp. + spp.; spp. + spp. + spp.; spp. + spp., however, spp., spp., spp., spp., spp. and spp. were practically absent (or in a low percentage) in the microbiome of women with SIL.
宫颈阴道微生物组的研究是医学科学中的一个关键研究领域。根据科学数据，存在于女性下生殖道的微生物可能会影响人乳头瘤病毒（HPV）的易感性和持续性，以及鳞状上皮内病变（SIL）向宫颈癌（CC）的发展和进程。
该研究纳入了67例经组织学诊断为低级别鳞状上皮内病变（LSIL）的患者和85例高级别鳞状上皮内病变（HSIL）的患者。采用16S rRNA基因测序方法检测了31例LSIL患者和45例HSIL患者的宫颈管微生物组，而其余36例LSIL患者和40例HSIL患者的微生物群落则采用PCR-RT进行分析。
HSIL患者的平均年龄比LSIL患者大3岁左右。HPV 16被发现是SIL患者中最常见的病毒类型。通过新一代测序确定育龄期SIL女性原核微生物的16S rRNA基因时，发现宫颈管微生物组中有许多非典型代表（土壤、水生和需氧菌）。除了属之外，其他属也可能包含在宫颈管的微生物组成中。失调紊乱在HSIL患者中更为常见。 属总是伴随着需氧性阴道炎（AV），而细菌性阴道病（BV）与 属和 属相关。比较这两种方法时， 属和 属可能在很大比例上包含在微生物生物量中。真杆菌属在16S测序方法中未出现。在RT-qPCR方法（Femoflor - 16）中，一些代表彼此同时被检测到，例如， 属 + 属 + 属； 属 + 属 + 属； 属 + 属，然而， 属、 属、 属、 属、 属和 属在SIL女性的微生物组中实际上不存在（或比例很低）。
