Rodrigues André Fellipe Freitas, Sales Maisa Vitória Santos, de Melo Gabriela Haro, da Silva Vinicio Berti, Mori Graziela Garrido
Medicine Course, University of Western São Paulo (UNOESTE), Presidente Prudente, Sao Paulo, Brazil.
Pharmacy Course, University of Western Sao Paulo(UNOESTE), Presidente Prudente, Sao Paulo, Brazil.
J Mol Neurosci. 2025 Sep 18;75(4):119. doi: 10.1007/s12031-025-02410-y.
The use of dyes is a common practice in several industrial sectors to increase the attractiveness of the merchandise. Tartrazine is a synthetic yellow dye used in various food products; it contains an azo bond in its composition. The present study aimed to analyze whether the chronic consumption of Tartrazine promotes changes in cognitive and behavioral parameters in vivo. A total of 40 Wistar rats (50% male and 50% female) were divided into three experimental groups: control group (CG) (n = 16), group treated with a dose within the acceptable daily intake (ADI), corresponding to 7.5 mg/kg/day (NEG) (n = 16), and group treated above the recommended value at a dose of 15 mg/kg/day (AEG) (n = 8). Tartrazine was administered via gavage daily for 10 months. Finally, the rats were subjected to the open field test (OFT), elevated plus maze (EPM), and object recognition test (OR) to evaluate their motor activity, anxiety, and long-term memory, respectively. All data were subjected to statistical analysis using ANOVA or Kruskal-Wallis tests, with a significance level of 5% (p < 0.05). There were no statistically significant changes in locomotor behavior (p = 0.351) and anxiety levels (p = 0.305) of the animals in either group. However, the group treated within the ADI demonstrated impairment in long-term memory (p < 0.001). Given this, it can be considered that prolonged use of Tartrazine, even within the ADI value limits, can cause mild impairment of certain cognitive functions.
在多个工业领域，使用染料是一种常见做法，目的是提高商品的吸引力。柠檬黄是一种用于各种食品的合成黄色染料；其成分中含有一个偶氮键。本研究旨在分析长期食用柠檬黄是否会在体内促进认知和行为参数的变化。总共40只Wistar大鼠（50%为雄性，50%为雌性）被分为三个实验组：对照组（CG）（n = 16）、接受每日可接受摄入量（ADI）剂量治疗的组，相当于7.5毫克/千克/天（NEG）（n = 16），以及接受高于推荐值剂量15毫克/千克/天治疗的组（AEG）（n = 8）。通过灌胃方式每日给予柠檬黄，持续10个月。最后，对大鼠进行旷场试验（OFT）、高架十字迷宫试验（EPM）和物体识别试验（OR），分别评估它们的运动活动、焦虑和长期记忆。所有数据均使用方差分析（ANOVA）或克鲁斯卡尔 - 沃利斯检验进行统计分析，显著性水平为5%（p < 0.05）。两组动物的运动行为（p = 0.351）和焦虑水平（p = 0.305）均无统计学显著变化。然而，接受ADI剂量治疗的组在长期记忆方面表现出损伤（p < 0.001）。鉴于此，可以认为即使在ADI值范围内长期使用柠檬黄，也可能导致某些认知功能的轻度损伤。
