Ghaneialvar Hori, Soltani Leila, Mami Sajad, Azizian Hajar, Abbasi Naser, Gholami Milad, Molaei Somayeh, Ghadermazi Mohammad, Alizadeh Reza, Akbari Amir Reza, Yari Milad, Soltani Salman
Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Department of Animal Sciences, Faculty of Agriculture, Razi University, Kermanshah, Iran.
Cell Biochem Funct. 2025 Sep;43(9):e70121. doi: 10.1002/cbf.70121.
In the present study, the synthesis, characterization, and therapeutic effects in treating second-degree burn wounds of the green synthesis ZnO NPs using Ferulago angulata extract and its doping with magnesium were evaluated. The prepared materials were characterized using powder X-ray diffraction, field emission scanning electron microscopy, UV-Vis absorption spectra, and Fourier transform infrared spectroscopy. The antioxidant properties of materials were investigated by DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay. The concentration of 500 µg/mL of Mg-doped ZnO NPs was associated with the highest level of DPPH radical reduction among the several substances (F. angulata extract, ZnO NPs, and Mg-doped ZnO NPs) (p < 0.05). The Pasteur Institute cell bank was employed to obtain the Huvec cell line for the cytotoxicity test. The viability of the cells decreased as compared to the control group (p < 0.05) after 24 h of exposure. The lowest viability rate was seen when these substances were added at 1000 µg/mL. The antibacterial and antifungal properties of nanoparticles and ointments prepared from these nanoparticles with different concentrations were investigated. Examining the antibacterial and antifungal effect of zinc oxide and Mg-doped ZnO nanoparticles showed that these compounds had antibacterial and antifungal effects on mesophilic aerobic bacteria, Escherichia coli, Pseudomonas aerogenosa, Staphylococcus aureus, Salmonella, Candida albicans, mold, and yeast. After 21 days of treatment with various ointments, the speed of wound closure and hair growth was observed in groups Mg-doped ZnO NPs (0.05% and 1%), Zn-NPs (0.05% and 1%), Adib Derm and Eucerin groups, respectively. The microscopic analysis of the wound site in the group treated with 0.1% of Mg-doped ZnO NPs showed that after 21 days, collagenization and epithelialization occurred. The epidermis and the stratum corneum were well-formed, and the formation of hair follicles increased in number. The presence of sebaceous glands around the hair follicles also indicated complete wound healing and improved skin.
在本研究中，评估了使用阿魏提取物绿色合成氧化锌纳米颗粒及其镁掺杂的合成、表征以及对二度烧伤创面的治疗效果。使用粉末X射线衍射、场发射扫描电子显微镜、紫外可见吸收光谱和傅里叶变换红外光谱对制备的材料进行表征。通过DPPH（2,2-二苯基-1-苦基肼）测定法研究材料的抗氧化性能。在几种物质（阿魏提取物、氧化锌纳米颗粒和镁掺杂氧化锌纳米颗粒）中，500µg/mL的镁掺杂氧化锌纳米颗粒浓度与最高水平的DPPH自由基还原相关（p<0.05）。采用巴斯德研究所细胞库获得用于细胞毒性测试的人脐静脉内皮细胞系。暴露24小时后，与对照组相比，细胞活力下降（p<0.05）。当以1000µg/mL添加这些物质时，观察到最低的活力率。研究了不同浓度的纳米颗粒以及由这些纳米颗粒制备的软膏的抗菌和抗真菌性能。检查氧化锌和镁掺杂氧化锌纳米颗粒的抗菌和抗真菌效果表明，这些化合物对嗜温需氧菌、大肠杆菌、产气假单胞菌、金黄色葡萄球菌、沙门氏菌、白色念珠菌、霉菌和酵母菌具有抗菌和抗真菌作用。用各种软膏治疗21天后，分别在镁掺杂氧化锌纳米颗粒组（0.05%和1%）、锌纳米颗粒组（0.05%和1%）、阿迪布皮肤软膏组和优色林组观察到伤口愈合速度和毛发生长情况。用0.1%的镁掺杂氧化锌纳米颗粒治疗组伤口部位的显微镜分析表明，21天后发生了胶原化和上皮化。表皮和角质层形成良好，毛囊数量增加。毛囊周围皮脂腺的存在也表明伤口完全愈合且皮肤得到改善。
