Combined effects of hyperbaric oxygen and ozone therapy on biochemical markers in ischemia-reperfusion injury: An experimental study.

OBJECTIVE

This study aimed to compare the effects of hyperbaric oxygen therapy (HBO) and ozone therapy (OT) on ischemia-reperfusion injury in Sprague-Dawley rat rectus abdominis muscle (RAM) flaps through the analysis of oxidative stress markers.

METHODS

This experimental study involved 36 adult Sprague-Dawley rats divided into five groups: HBO, OT, HBO + OT, Control, and Sham. Ischemia-reperfusion injury was induced in RAM flaps for 4 h. Levels of myeloperoxidase (MPO), superoxide dismutase (SOD), and malondialdehyde (MDA) were measured in serum samples on the 1st and 7th days using the ELISA method.

RESULTS

SOD levels increased significantly on the 7th day in the HBO + OT group (p = 0.044). MPO levels were significantly elevated in the Sham and Control groups by day 7 (p = 0.049, p = 0.025). MDA levels increased significantly in the Control, OT, and HBO groups on day 7 (p = 0.028). While HBO and OT reduced oxidative stress, the combined HBO + OT therapy appeared to elevate oxidative stress markers over time.

CONCLUSION

HBO and OT therapies show individual efficacy in reducing oxidative stress during ischemia-reperfusion injury. While both therapies reduced oxidative damage, combining them might offer additional protection.