Farag Amrou, Sharma Dushyant
Diabetes and Endocrinology, Royal Liverpool University Hospital, Liverpool, GBR.
Diabetes and Endocrinology, Liverpool University Hospitals NHS Foundation Trust, Liverpool, GBR.
Cureus. 2025 Sep 19;17(9):e92683. doi: 10.7759/cureus.92683. eCollection 2025 Sep.
As our understanding of vitamin D and its metabolites has improved, different assays have been developed for measuring their serum concentrations. The predominant circulating metabolite, 25-hydroxyvitamin D (25(OH)D), has been extensively studied and is now widely used for assessing vitamin D status. Of current interest is the biologically active metabolite, 1,25-dihydroxyvitamin D (1,25(OH)D), which is involved in numerous physiological processes related to bone metabolism and various non-calcaemic functions. Quantification of 1,25(OH)D serves not only as a diagnostic tool for numerous diseases, but also as a way to ensure optimal levels in the circulation to maintain physiological functioning. Measuring 1,25(OH)D, however, proves challenging as its concentration in the blood is very low, and the molecule itself is highly lipophilic. A variety of analytical assays have been developed for measuring 1,25(OH)D, each differing in its analytical principles. These methods exhibit variations in their performances, showcasing both strengths and limitations in their underlying analytical principles; some of these assays continue to be used today, while others have become obsolete. Comparability of these methods to this day is challenging due to the absence of a standardisation scheme for 1,25(OH)D.
随着我们对维生素D及其代谢产物的了解不断深入，已开发出不同的检测方法来测量它们在血清中的浓度。主要的循环代谢产物25-羟基维生素D（25(OH)D）已得到广泛研究，目前被广泛用于评估维生素D状态。目前受到关注的是具有生物活性的代谢产物1,25-二羟基维生素D（1,25(OH)D），它参与了许多与骨代谢相关的生理过程以及各种非钙调节功能。对1,25(OH)D进行定量不仅可作为多种疾病的诊断工具，还可作为确保其在循环中达到最佳水平以维持生理功能的一种方式。然而，测量1,25(OH)D被证明具有挑战性，因为其在血液中的浓度非常低，而且该分子本身具有高度亲脂性。已开发出多种分析方法来测量1,25(OH)D，每种方法的分析原理都有所不同。这些方法在性能上存在差异，在其潜在的分析原理方面既展示了优势也存在局限性；其中一些检测方法至今仍在使用，而其他一些已被淘汰。由于缺乏针对1,25(OH)D的标准化方案，时至今日这些方法之间的可比性仍具有挑战性。
