Zin Eldin Asmaa I, Hozyen Heba F, Shafik Eman, Eissa Nourhan
Department of Microbiology and Immunology, National Research Center (NRC), Giza, Egypt.
Department of Animal Reproduction and AI, National Research Center (NRC), Giza, Egypt.
Open Vet J. 2025 Jun;15(6):2518-2531. doi: 10.5455/OVJ.2025.v15.i6.24. Epub 2025 Jun 30.
Cattle mastitis is a widespread and affluent illness that threatens the dairy industry. Numerous common infectious diseases, mostly zoonotic, impact Egypt's dairy cow production. According to their remarkable affordability, high safety, and biocompatibility, and their exceptional effectiveness against microbes, zinc oxide nanoparticles (ZnO-NPs) have shown great promise in biomedicine, particularly in the areas of antibacterial and anticancer treatment, and so have become the most broadly used nanoparticles of metal oxides in various biological treatments throughout the last 20 years.
The present study emphasized the potential for dispersed, non-agglomerated ZnO-NPs produced by sonochemical irradiation with starch serving as a capping agent to be considered more economical and effective germicidal agents in the eradication of bacteria of bovine mastitis, such as serovar Typhimurium ( Typhimurium), and .
To improve their antibacterial effectiveness against specific zoonotic pathogens that can cause bovine mastitis, three suspensions of ZnO-NPs were created as follows: auto-combustion reaction-synthesized ZnO-NPs, auto-combustion reaction-prepared uncapped ZnO-NPs, and sonochemically prepared starch-capped ZnO-NPs. The three suspensions were exposed to the zoonotic pathogens Typhimurium, and E. faecium at concentrations ranging from 1 to 50 mg/ml.
The prepared three nano-ZnO suspensions showed minimum bactericidal concentration (MBC) total suppression of at doses of 1-10 mg/ml of nano-ZnO and minimum inhibitory concentration (MIC) against at concentrations <1-5 mg/ml. According to the suspension (No. 1), MBC was detected at a concentration of 30 mg/ml, and the MIC was 20 mg/ml for . Concerning Typhimurium, the MIC was found at a concentration of 5 mg/ml, while MBC was measured at 10 mg/ml. Lastly, at a concentration of 30 mg/ml, the nano-ZnO suspension (No. 1) demonstrated MBC activity against , with MIC activity at a concentration of 2 mg/ml.
This point will be useful for future research on ZnO-NPs because it focuses on their biological and antibacterial applications.
奶牛乳腺炎是一种广泛存在且影响严重的疾病，威胁着乳制品行业。许多常见的传染病，大多是人畜共患病，影响着埃及的奶牛生产。氧化锌纳米颗粒（ZnO-NPs）因其价格低廉、安全性高、生物相容性好以及对微生物具有卓越的抗菌效果，在生物医学领域，尤其是抗菌和抗癌治疗方面展现出巨大潜力，在过去20年里已成为各种生物治疗中应用最广泛的金属氧化物纳米颗粒。
本研究着重探讨以淀粉为封端剂通过声化学辐照制备的分散、非团聚的ZnO-NPs作为更经济有效的杀菌剂用于根除奶牛乳腺炎细菌（如鼠伤寒血清型菌（鼠伤寒菌）和粪肠球菌）的潜力。
为提高其对可引起奶牛乳腺炎的特定人畜共患病原体的抗菌效果，制备了三种ZnO-NPs悬浮液，如下：自燃烧反应合成的ZnO-NPs、自燃烧反应制备的未封端ZnO-NPs以及声化学制备的淀粉封端ZnO-NPs。将这三种悬浮液分别以1至50mg/ml的浓度暴露于人畜共患病原体鼠伤寒菌和粪肠球菌。
制备的三种纳米氧化锌悬浮液在纳米氧化锌剂量为1 - 10mg/ml时对粪肠球菌显示出最小杀菌浓度（MBC）完全抑制，在浓度<1 - 5mg/ml时对鼠伤寒菌显示出最小抑菌浓度（MIC）。根据悬浮液（1号），检测到对粪肠球菌的MBC浓度为30mg/ml，MIC为20mg/ml。对于鼠伤寒菌，MIC浓度为5mg/ml，而MBC为10mg/ml。最后，在浓度为30mg/ml时，纳米氧化锌悬浮液（1号）对粪肠球菌表现出MBC活性，在浓度为2mg/ml时表现出MIC活性。
这一点将有助于未来关于ZnO-NPs的研究，因为它聚焦于其生物学和抗菌应用。
