使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐法比较一种新型充填材料与Metapex用于乳牙牙髓摘除术的细胞毒性：一项研究。

Comparison of the Cytotoxicity of a Novel Obturating Material with Metapex for Pulpectomy in Primary Teeth Using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide Assay: An Study.

作者信息

Rajadurai Manisha Bala Rathy, Govindaraju Lavanya

机构信息

Department of Pediatric and Preventive Dentistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.

出版信息

Int J Clin Pediatr Dent. 2025 Aug;18(8):917-921. doi: 10.5005/jp-journals-10005-3167. Epub 2025 Sep 4.

DOI:10.5005/jp-journals-10005-3167

PMID:40989990

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12451584/

Abstract

INTRODUCTION

Pulpectomy is a critical procedure for managing pulpally infected primary teeth. The choice of obturating material is important for the treatment's success. Metapex, a commonly used material, has several disadvantages such as rapid resorption and resistance issues against certain microorganisms. To overcome these disadvantages, a novel obturating material combining zinc oxide, calcium hydroxide, and metronidazole was developed. The aim of the study is to compare cytotoxic effects of the novel obturating material with Metapex using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.

MATERIALS AND METHODS

An study was conducted using 3T3 mouse fibroblast cells. The cells were treated with different concentrations (2.5, 5, 10, 20, 40, 60 µg/mL) of the novel obturating material and Metapex. Cell viability was assessed using the MTT assay, where formazan crystals were dissolved in DMSO and absorbance was measured at 570 nm. Independent -tests were used to compare cell viability between the groups, while repeated measures analysis of variance (ANOVA) with Bonferroni tests analyzed intragroup differences.

RESULTS

Both materials demonstrated minimal cytotoxicity at lower concentrations (2.5-10 µg/mL) and progressively higher cytotoxicity at increased concentrations (20-60 µg/mL). In the novel material group, significant differences were observed between 2.5 and 5 µg/mL ( = 0.015). However, independent -tests revealed no significant differences between the two materials across various concentrations ( > 0.05).

CONCLUSION

The novel obturating material showed acceptable cytotoxicity at lower concentrations but exhibited higher cytotoxicity at increased levels. Further research is needed to optimize its composition for safe clinical use, particularly in pediatric patients.

HOW TO CITE THIS ARTICLE

Rajadurai MBR, Govindaraju L. Comparison of the Cytotoxicity of a Novel Obturating Material with Metapex for Pulpectomy in Primary Teeth Using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide Assay: An Study. Int J Clin Pediatr Dent 2025;18(8):917-921.

摘要

引言

牙髓摘除术是治疗乳牙牙髓感染的关键步骤。充填材料的选择对治疗成功与否至关重要。常用材料Metapex存在一些缺点，如吸收迅速以及对某些微生物的抵抗问题。为克服这些缺点，研发了一种新型充填材料，其由氧化锌、氢氧化钙和甲硝唑组成。本研究的目的是使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）试验比较新型充填材料与Metapex的细胞毒性作用。

材料与方法

使用3T3小鼠成纤维细胞进行一项研究。用不同浓度（2.5、5、10、20、40、60μg/mL）的新型充填材料和Metapex处理细胞。使用MTT试验评估细胞活力，将甲臜晶体溶解于二甲基亚砜（DMSO）中，并在570nm处测量吸光度。采用独立样本t检验比较各组之间的细胞活力，而使用重复测量方差分析（ANOVA）和Bonferroni检验分析组内差异。

结果

两种材料在较低浓度（2.5 - 10μg/mL）时均表现出最小的细胞毒性，而在浓度增加时（20 - 60μg/mL）细胞毒性逐渐增大。在新型材料组中，观察到2.5μg/mL和5μg/mL之间存在显著差异（P = 0.015）。然而，独立样本t检验显示两种材料在不同浓度下均无显著差异（P > 0.05）。