Adachi Kosei, Hyodo Fuminori, Elhelaly Abdelazim Elsayed, Ichihashi Koki, Mori Takashi, Tomita Hiroyuki, Mori Takayuki, Imai Hirohiko, Matsuo Masayuki
Department of Radiology, Graduate School of Medicine, Gifu University, Gifu, Gifu, Japan.
Department of Radiology, Asahi University Hospital, Gifu, Gifu, Japan.
Discov Nano. 2025 Sep 24;20(1):166. doi: 10.1186/s11671-025-04355-y.
The intestine is an important organ in radiotherapy of the abdominal region, and radiation-induced intestinal injury (RIII) is an undesirable biological response to radiotherapy. Radiotherapy is known to induce oxidative stress associated with the generation of reactive oxygen species (ROS), which in turn plays an important role in RIII. However, these effects cannot be detected or predicted early using conventional imaging techniques such as computed tomography and magnetic resonance imaging (MRI). In this study, an intestinal redox imaging method using dynamic nuclear polarization (DNP) MRI and carbamoyl PROXYL (CmP) was designed. The probe was prepared in a solution of increased viscosity of the CmP solution that is not affected by peristalsis. This redox imaging method enabled noninvasive redox imaging of the intestine and detection of RIII at an early stage of progression. Our findings suggest that redox imaging can aid in monitoring early metabolic changes that occur during the pathogenesis of this condition.
肠道是腹部放疗中的重要器官，辐射诱导的肠道损伤（RIII）是放疗中不良的生物学反应。已知放疗会诱导与活性氧（ROS）生成相关的氧化应激，而氧化应激在RIII中起重要作用。然而，使用计算机断层扫描和磁共振成像（MRI）等传统成像技术无法早期检测或预测这些效应。在本研究中，设计了一种使用动态核极化（DNP）MRI和氨基甲酰基PROXYL（CmP）的肠道氧化还原成像方法。该探针在不受蠕动影响的CmP溶液的高粘度溶液中制备。这种氧化还原成像方法能够对肠道进行无创氧化还原成像，并在疾病进展的早期阶段检测到RIII。我们的研究结果表明，氧化还原成像有助于监测这种疾病发病机制中发生的早期代谢变化。
