Duran-Ahumada Sebastian, Petersen Vivian, Futo Michael, Zamy Mathieu, Pereira Timothy, Burini Bianca C
Florida Medical Entomology Laboratory, University of Florida, Vero Beach, FL, 32962, USA.
Department of Entomology, Minnie Belle Heep Center, Texas A & M University, College Station, TX, 77843-2475, USA.
Parasit Vectors. 2025 Sep 24;18(1):389. doi: 10.1186/s13071-025-07008-2.
The work of collecting mosquitoes in the field is essential for improving knowledge about species of known and unknown vector status, their invasion dynamics, and further understanding their involvement in circulating pathogens of medical and veterinary importance. Over the years, different techniques have been developed to capture mosquitoes for entomological, pathogen transmission, and surveillance studies. For entomological and transmission dynamic research to be reliable, it is essential for mosquito specimens to be correctly identified so that their role in pathogen transmission can be appropriately assessed. Currently, modern molecular techniques support traditional morphological taxonomic identification and provide faster and more assertive identification. It is also important to test the susceptibility of potentially relevant mosquitoes or mosquitoes with unknown vector status in the laboratory to identify which species could participate in pathogen transmission. This last step toward fully performing vector incrimination of mosquito species and understanding their interactions with relevant pathogens requires proper handling of live specimens and laboratory colonization under artificial conditions to perform artificial infection studies. In this work, we aim to underscore the significance of the available tools for entomological studies and pathogen transmission research while also offering insights into the principles behind recent technological advancements that enhance the effectiveness and reliability of these studies.
在野外收集蚊子的工作对于增进对已知和未知媒介地位物种、其入侵动态的了解，以及进一步了解它们在传播具有医学和兽医学重要性的病原体方面的作用至关重要。多年来，已经开发出不同的技术来捕获蚊子用于昆虫学、病原体传播和监测研究。为了使昆虫学和传播动态研究可靠，正确识别蚊子标本至关重要，以便能够适当地评估它们在病原体传播中的作用。目前，现代分子技术支持传统的形态分类鉴定，并提供更快、更可靠的鉴定。在实验室中测试潜在相关蚊子或媒介地位未知的蚊子的易感性也很重要，以确定哪些物种可能参与病原体传播。朝着全面确定蚊子物种的媒介作用并了解它们与相关病原体的相互作用的最后一步，需要在人工条件下妥善处理活标本并进行实验室定殖，以开展人工感染研究。在这项工作中，我们旨在强调现有工具在昆虫学研究和病原体传播研究中的重要性，同时也深入探讨最近技术进步背后的原理，这些进步提高了这些研究的有效性和可靠性。
