He Haitong, Liu Weiqing, Xu Yiwei, Fang Xuerui, Zhang Wei, Kong Zhaosheng, Wang Lixiang
Shanxi Hou Ji Laboratory, College of Agriculture, Shanxi Agricultural University, Taigu, 030801 China.
aBIOTECH. 2025 Jul 2;6(3):542-553. doi: 10.1007/s42994-025-00222-7. eCollection 2025 Sep.
Peanut () is a widely cultivated legume crop that can fix nitrogen by forming root nodules with compatible rhizobia. The initiation and formation of these nodules require complex molecular communication between legumes and rhizobia, involving the precise regulation of multiple legume genes. However, the mechanism underlying nodulation in peanuts remains poorly understood. In this study, we identified a gene associated with nodulation in peanuts, named (). Multiple lines of evidence indicate that is primarily expressed in peanut nodules. Silencing or knocking out in peanut resulted in fewer nodules, as well as lower fresh weight and nitrogenase activity, while overexpressing significantly enhanced nodulation ability and nitrogenase activity. Modulating the expression of also influenced the expression levels of genes associated with the Nod factor signaling pathway and infection via crack entry. Comparative transcriptome analysis revealed that likely regulates peanut nodulation by affecting the expression of genes involved in the cytokinin and calcium signaling pathways. Our data thus show that acts as a crucial regulator promoting symbiotic nodulation in peanuts.
The online version contains supplementary material available at 10.1007/s42994-025-00222-7.
花生（）是一种广泛种植的豆科作物，它可以通过与相容的根瘤菌形成根瘤来固氮。这些根瘤的起始和形成需要豆科植物与根瘤菌之间复杂的分子通讯，涉及多个豆科植物基因的精确调控。然而，花生结瘤的潜在机制仍知之甚少。在本研究中，我们鉴定了一个与花生结瘤相关的基因，命名为（）。多项证据表明，主要在花生根瘤中表达。在花生中沉默或敲除会导致根瘤数量减少，以及鲜重和固氮酶活性降低，而过量表达则显著增强结瘤能力和固氮酶活性。调节的表达也会影响与根瘤因子信号通路和通过裂缝侵入感染相关的基因的表达水平。比较转录组分析表明，可能通过影响细胞分裂素和钙信号通路中相关基因的表达来调节花生结瘤。因此，我们的数据表明，在花生中作为促进共生结瘤的关键调节因子发挥作用。
在线版本包含可在10.1007/s42994-025-00222-7获取的补充材料。
