Liu Chang, Cui Baiyu, Hu Lian, Li Rui, Xiao Peng, Zuo Jun, Wang Zeshuang, Ma Zengling, Fan Yuping, Zhang He, Li Renhui
Zhejiang Provincial Key Laboratory for Subtropical Water Environment and Marine Biological Resources Protection, National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution, College of Life and Environmental Sciences, Wenzhou University, Wenzhou, China.
Wenzhou Shanxi Hydro-junction Management Center, Wenzhou, China.
Front Microbiol. 2025 Sep 9;16:1623621. doi: 10.3389/fmicb.2025.1623621. eCollection 2025.
Harmful cyanobacterial blooms occur frequently worldwide with serious environmental impacts. Many detrimental cyanobacteria are well known for their potential to synthesize various cyanotoxins, posing significant threats to aquatic ecosystems and public health worldwide. While most studies focus on the toxicological impacts of microcystins and its main producer , the ecotoxic effects of anatoxin-a and anatoxin-a-producing cyanobacteria are not fully understood. This study investigated the response of zebrafish () to a ruptured cell solution (RCS) of a planktonic anatoxin-a-producing . The RCS exposure negatively affected the hatching of zebrafish embryos, and promoted malformation. Furthermore, RCS treatment also disrupted neurobehaviors, and induced severe oxidative stress. In addition, the RCS inhibited the activity of acetylcholinesterase, and dysregulated the expression of several genes related to neuron-development and activated apoptosis in zebrafish. These results suggest that blooms of anatoxin-a-producing will have neurotoxic effects on aquatic animals, and could impact human health. They help to further understand the potential neurotoxicity of harmful cyanobacteria, and highlight the need for further studies and risk assessments of the ecological impacts of harmful cyanobacterial blooms in freshwater ecosystems.
有害蓝藻水华在全球频繁发生，具有严重的环境影响。许多有害蓝藻以其合成各种蓝藻毒素的潜力而闻名，对全球水生生态系统和公众健康构成重大威胁。虽然大多数研究集中在微囊藻毒素及其主要产生者的毒理学影响，但对类毒素-a和产类毒素-a蓝藻的生态毒理学效应尚未完全了解。本研究调查了斑马鱼对浮游产类毒素-a蓝藻的破裂细胞溶液（RCS）的反应。RCS暴露对斑马鱼胚胎的孵化产生负面影响，并促进畸形。此外，RCS处理还扰乱了神经行为，并诱导了严重的氧化应激。此外，RCS抑制了乙酰胆碱酯酶的活性，并使斑马鱼中与神经元发育相关的几个基因的表达失调，并激活了细胞凋亡。这些结果表明，产类毒素-a蓝藻的水华将对水生动物产生神经毒性作用，并可能影响人类健康。它们有助于进一步了解有害蓝藻的潜在神经毒性，并强调需要对淡水生态系统中有害蓝藻水华的生态影响进行进一步研究和风险评估。
