Wexler B C, Judd J T
Br J Exp Pathol. 1970 Dec;51(6):646-52.
Arteriosclerotic and non-arteriosclerotic, male Sprague-Dawley rats were given 2 s.c. injections of isoproterenol known to produce extensive myocardial infarction. The appearance of positive fuchsinophilia was used as an index of focal myocardial acidosis and of anaerobic metabolism. After one injection of isoproterenol, positive fuchsinophilia appeared within 30 min., reached a zenith at 4 hr and then promptly disappeared. Following the second injection of isoproterenol, fuchsinophilia reappeared briefly but was not as intense. The arteriosclerotic animals showed markedly less evidence of heart failure outwardly and less evidence of fuchsinophilia, histopathologically. Apparently, the first episode of cardiac stimulation caused only temporary cardiac ischaemia, positive fuchsinophilia and anaerobic cardiac metabolism. After the second injection, however, irreversible cardiac damage occurred and despite an abortive attempt towards anaerobic metabolic readjustment overt cardiac necrosis became dominant.
对雄性斯普拉格 - 道利大鼠，分为动脉粥样硬化组和非动脉粥样硬化组，皮下注射两次异丙肾上腺素，已知该药物可导致广泛的心肌梗死。将品红嗜染阳性的出现作为局灶性心肌酸中毒和无氧代谢的指标。注射一次异丙肾上腺素后，品红嗜染阳性在30分钟内出现，4小时达到高峰，然后迅速消失。第二次注射异丙肾上腺素后，品红嗜染短暂再现，但程度不如第一次。从外表看，动脉粥样硬化动物的心力衰竭迹象明显较少，从组织病理学上看，品红嗜染的迹象也较少。显然，第一次心脏刺激仅引起暂时性心肌缺血、品红嗜染阳性和心脏无氧代谢。然而，第二次注射后，发生了不可逆的心脏损伤，尽管试图进行无氧代谢重新调整但未成功，明显的心脏坏死占了主导。
