直接对血培养物使用blaZ定量聚合酶链反应检测金黄色葡萄球菌基因型青霉素敏感性，结果显示与表型高度一致。
Staphylococcus aureus genotypic penicillin susceptibility using a blaZ qPCR directly on blood cultures shows high concordance with phenotype.
作者信息
Vang Tobias Müller, Holzknecht Barbara Juliane, Nilsson Stephen Strunge, Hoppe Morten, Pedersen Michael, Krogfelt Karen Angeliki, Eriksen Helle Brander
机构信息
Department of Clinical Microbiology, Copenhagen University Hospital - Herlev and Gentofte, Herlev, Denmark.
Department of Science and Environment, Roskilde university, Roskilde, Denmark.
出版信息
Eur J Clin Microbiol Infect Dis. 2025 Sep 25. doi: 10.1007/s10096-025-05272-9.
Staphylococcus aureus blood cultures were investigated geno- and phenotypically for penicillin susceptibility. A qPCR method detecting the blaZ gene was performed directly on positive blood culture vials and compared with routine disk diffusion results supplemented with the cloverleaf test. Among the collected vials, 100 were blaZ positive and 101 were blaZ negative. Full concordance was observed between genotypical and phenotypical penicillin susceptibility, however one penicillin resistant isolate was only weakly phenotypically positive.
对金黄色葡萄球菌血培养物进行了青霉素敏感性的基因和表型研究。直接在阳性血培养瓶上进行检测blaZ基因的qPCR方法，并与补充了三叶草试验的常规纸片扩散结果进行比较。在所收集的培养瓶中，100个为blaZ阳性，101个为blaZ阴性。在基因型和表型青霉素敏感性之间观察到完全一致，然而，一株耐青霉素分离株仅表现出弱阳性表型。
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