淋巴细胞表面的免疫球蛋白。I. 分布与定量
Immunoglobulins on the surface of lymphocytes. I. Distribution and quantitation.
作者信息
Rabellino E, Colon S, Grey H M, Unanue E R
出版信息
J Exp Med. 1971 Jan 1;133(1):156-67. doi: 10.1084/jem.133.1.156.
The distribution, and quantity of immunoglobulins on the surface of lymphocytes has been studied by means of immunofluorescence and a quantitative radio-immunoassay. Surface immunoglobulins were found on approximately 45% of spleen and marrow lymphocytes and 7-14% of lymphocytes from lymph nodes, peripheral blood, and peritoneal exudate. Thymic lymphocytes contained undetectable amounts of immunoglobulin. In the spleen the different immunoglobulins were present in the following order: gammaG2 > gammaG1 > M > gammaA > gammaG3. The surface immunoglobulin was largely removable by brief treatment with trypsin. Quantitative analysis indicated that 50,000-150,000 molecules of immunoglobulin were present on an individual cell. A variety of observations make it likely that this lymphocyte-associated immunoglobulin. is a product of the cell to which it is attached rather than a form of cytophilic antibody.
已通过免疫荧光法和定量放射免疫测定法研究了淋巴细胞表面免疫球蛋白的分布及数量。在约45%的脾脏和骨髓淋巴细胞以及7%-14%的来自淋巴结、外周血和腹腔渗出液的淋巴细胞表面发现了表面免疫球蛋白。胸腺淋巴细胞所含免疫球蛋白量检测不到。在脾脏中，不同免疫球蛋白的存在顺序如下：γG2>γG1>M>γA>γG3。表面免疫球蛋白经胰蛋白酶短暂处理后大部分可去除。定量分析表明，单个细胞上存在50,000-150,000个免疫球蛋白分子。各种观察结果表明，这种与淋巴细胞相关的免疫球蛋白很可能是其所附着细胞的产物，而非亲细胞抗体的一种形式。
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