Byrt P, Ada G L
Immunology. 1969 Oct;17(4):503-16.
Labelled bacterial flagellin and haemocyanin reacted with lymphocyte-like cells from several rat and mouse tissues. This reaction occurred at low temperatures and in the presence of sodium azide, conditions which inhibited uptake of labelled proteins by phagocytic cells. The reactive cells took up at least 40,000 molecules of labelled flagellin, and appeared to have a much greater capacity, since pretreatment with 10,000 times this amount of flagellin was required to inhibit the reaction. The flagellin and haemocyanin were firmly bound to the cells, possibly to immunoglobulin at the cell surface, since prior treatment with antisera directed against mouse immunoglobulin inhibited the reaction with lymphocytes from mouse spleen and peritoneal exudate. The number of reactive cells in spleen was proportional to the concentration of labelled protein. At a given concentration of labelled protein, however, the number of reactive lymphocytes was characteristic of each tissue. Spleen, lymph node and thoracic duct lymph contained a similar proportion of reactive lymphocyte-like cells, peritoneal exudate contained more, and thymus contained few if any such cells. Bone marrow contained a high proportion of reactive lymphoid cells which apparently reacted in a non-specific manner, since the uptake of labelled protein by these cells was not inhibited by anti-immunoglobulin serum.
标记的细菌鞭毛蛋白和血蓝蛋白与来自几只大鼠和小鼠组织的淋巴细胞样细胞发生反应。这种反应在低温和叠氮化钠存在的情况下发生，这些条件会抑制吞噬细胞对标记蛋白的摄取。反应性细胞摄取了至少40,000个标记的鞭毛蛋白分子，并且似乎具有更大的容量，因为需要用比该量多10,000倍的鞭毛蛋白进行预处理才能抑制反应。鞭毛蛋白和血蓝蛋白与细胞紧密结合，可能与细胞表面的免疫球蛋白结合，因为用针对小鼠免疫球蛋白的抗血清预先处理可抑制与小鼠脾脏和腹腔渗出液中淋巴细胞的反应。脾脏中反应性细胞的数量与标记蛋白的浓度成正比。然而，在给定的标记蛋白浓度下，反应性淋巴细胞的数量是每个组织的特征。脾脏、淋巴结和胸导管淋巴中含有相似比例的反应性淋巴细胞样细胞，腹腔渗出液中更多，而胸腺中几乎没有或根本没有此类细胞。骨髓中含有高比例的反应性淋巴细胞，这些细胞显然以非特异性方式反应，因为这些细胞对标记蛋白的摄取不受抗免疫球蛋白血清的抑制。
