Cerottini J C, Brunner K T
Immunology. 1967 Oct;13(4):395-403.
An immunofluorescent technique is described which permits the localization and the semi-quantitative evaluation of isoantigens. Studies of normal and tumour cells have demonstrated that H-2 isoantigens are located in discrete areas on the cell surface. The thymus of 20–25-day-old mice was found to contain approximately 85 per cent lymphoid cells with a very low isoantigen content. These cells are considered to represent thymus cortical lymphoid cells. Cortisol treatment of mice reduced the relative number of cells with low isoantigen content to 10 per cent.
本文描述了一种免疫荧光技术，该技术可用于定位和半定量评估同种抗原。对正常细胞和肿瘤细胞的研究表明，H-2同种抗原位于细胞表面的离散区域。发现20至25日龄小鼠的胸腺中约85%的淋巴细胞同种抗原含量非常低。这些细胞被认为代表胸腺皮质淋巴细胞。用皮质醇处理小鼠可使同种抗原含量低的细胞相对数量减少至10%。
