Zhan Jiyuan, Huang Yinxue, Meng Xinhui, Wang Yiquan, Liang Jia, Zhu Fengjiao, She Rui, Huang Shanshan, Huo Lijun
Yunnan Key Laboratory of Stomatology & Department of Cariology, Operative Dentistry and Endodontics, The Affiliated Stomatology Hospital, Kunming Medical University, Kunming, China.
J Oral Microbiol. 2025 Sep 23;17(1):2560016. doi: 10.1080/20002297.2025.2560016. eCollection 2025.
While the microbiome of caries-derived periapical lesions has been extensively characterized, the microbial profile of trauma-derived periapical lesions remains poorly understood. This study aimed to characterize the apical microbiome of trauma-derived periapical lesions and identify taxonomic differences between trauma- and caries-derived periapical lesions.
Twenty patients with periapical lesions were enrolled, comprising 10 trauma-derived cases (trauma group) and 10 caries-derived cases (caries group). Microbial samples were collected using sterile paper points inserted into the root canal exudate, followed by DNA extraction and Illumina sequencing of the hypervariable V3-V4 regions of the 16S rRNA gene. Bioinformatic analyses included -diversity, -diversity based on Bray-Curtis distance and differential abundance testing (LEfSe method with LDA score ≥ 2.0).
Sequencing revealed 36 bacterial phyla and 587 genera across all samples. Trauma group showed significantly greater relative abundance of ( = 0.002) compared to caries group, whereas ( = 0.008), ( = 0.041) and ( = 0.006) exhibited reduced abundance. The core microbiota in the trauma group included , and , based on relative abundance. LEfSe analysis identified as a biomarker for the trauma group.
Trauma-derived periapical lesions exhibited reduced microbial diversity compared to caries-derived periapical lesions, with identified as a potential pathognomonic taxon for trauma-derived periapical lesions.
虽然龋源性根尖周病变的微生物群已得到广泛表征，但创伤性根尖周病变的微生物特征仍知之甚少。本研究旨在表征创伤性根尖周病变的根尖微生物群，并确定创伤性和龋源性根尖周病变之间的分类学差异。
招募了20例根尖周病变患者，包括10例创伤性病例（创伤组）和10例龋源性病例（龋病组）。使用无菌纸尖插入根管渗出液中收集微生物样本，随后进行DNA提取和16S rRNA基因高变V3-V4区域的Illumina测序。生物信息学分析包括α多样性、基于Bray-Curtis距离的β多样性和差异丰度测试（LDA得分≥2.0的LEfSe方法）。
测序显示所有样本中共有36个细菌门和587个属。与龋病组相比，创伤组的[具体细菌名称1]相对丰度显著更高（P = 0.002），而[具体细菌名称2]（P = 0.008）、[具体细菌名称3]（P = 0.041）和[具体细菌名称4]（P = 0.006）的丰度降低。基于相对丰度，创伤组的核心微生物群包括[具体细菌名称5]、[具体细菌名称6]和[具体细菌名称7]。LEfSe分析确定[具体细菌名称8]为创伤组的生物标志物。
与龋源性根尖周病变相比，创伤性根尖周病变的微生物多样性降低，[具体细菌名称8]被确定为创伤性根尖周病变的潜在特征性分类群。
