在核酸分离过程中使用金精三羧酸作为核酸酶抑制剂。
Use of aurintricarboxylic acid as an inhibitor of nucleases during nucleic acid isolation.
作者信息
Hallick R B, Chelm B K, Gray P W, Orozco E M
出版信息
Nucleic Acids Res. 1977 Sep;4(9):3055-64. doi: 10.1093/nar/4.9.3055.
Aurintricarboxylic acid (ATA) is a general inhibitor of nucleases. ATA has been shown to inhibit the following enzymes in vitro: DNAse I, RNAse A, S1 nuclease, exonuclease III, and restriction endonucleases Sal I, Bam HI, Pst I and Sma I. The observed inhibition is consistent with the proposal by Blumenthal and Landers (BBRC 55, 680, 1973) that most nucleic acid binding proteins will be sensitive to ATA. The action of ATA as a nuclease inhibitor can be used to advantage in the isolation of cellular nucleic acids.
金精三羧酸（ATA）是一种核酸酶的通用抑制剂。已证明ATA在体外可抑制以下酶：脱氧核糖核酸酶I、核糖核酸酶A、S1核酸酶、核酸外切酶III以及限制性内切酶Sal I、Bam HI、Pst I和Sma I。观察到的抑制作用与布卢门撒尔和兰德斯（《生物化学与生物物理研究通讯》55, 680, 1973）提出的大多数核酸结合蛋白对ATA敏感的观点一致。ATA作为核酸酶抑制剂的作用可用于细胞核酸的分离。
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