金精三羧酸，一种蛋白质合成起始的优先抑制剂。
Aurintricarboxylic acid, a preferential inhibitor of initiation of protein synthesis.
作者信息
Siegelman F, Apirion D
出版信息
J Bacteriol. 1971 Mar;105(3):902-7. doi: 10.1128/jb.105.3.902-907.1971.
Abstract
The effect of aurintricarboxylic acid (ATA) was tested on various aspects of protein synthesis directed by the natural messenger ribonucleic acid (RNA) isolated from R17 RNA bacteriophage. The effects of various levels of ATA (up to 1,000 mum) were tested on overall protein synthesis as well as on binding of messenger RNA and fmet-transfer RNA to ribosomes and on the addition of the 50S ribosome to the 30S ribosome initiation complex. All of the reactions tested could be inhibited by ATA, and none of the tested steps was found to be uniquely sensitive to it. However, the total initiation steps were more sensitive to this chemical than the elongation steps; thus, under appropriate conditions this chemical can preferentially inhibit initiation while elongation of the polypeptide chain is not appreciably affected.
摘要
测试了金精三羧酸（ATA）对由从R17 RNA噬菌体分离的天然信使核糖核酸（RNA）指导的蛋白质合成各个方面的影响。测试了不同水平的ATA（高达1000μm）对整体蛋白质合成以及信使RNA和甲硫氨酸起始转运RNA与核糖体的结合以及50S核糖体添加到30S核糖体起始复合物的影响。所有测试的反应都可被ATA抑制，并且未发现任何测试步骤对其具有独特的敏感性。然而，总的起始步骤比延伸步骤对这种化学物质更敏感；因此，在适当条件下，这种化学物质可优先抑制起始，而多肽链的延伸不受明显影响。
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Inhibition of transfer factor Ts by aurintricarboxylic acid.
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