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从生物标志物到生物传感器：检测基质金属蛋白酶（MMPs）的现代方法

From Biomarkers to Biosensors: Modern Approaches for the Detection of Matrix Metalloproteinases (MMPs).

作者信息

Chinnappan Raja, Ramachandran Lohit, Uttam Isha, Citartan Marimuthu, Ballal Nidambur Vasudev, Mani Naresh Kumar

机构信息

College of Medicine, Alfaisal University, Riyadh 11533, Saudi Arabia.

Microfluidics, Sensors and Diagnostics (μSenD) Laboratory, Centre for Microfluidics, Biomarkers, Photoceutics and Sensors (µBioPS), Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, Udupi 576104, Karnataka, India.

出版信息

Biosensors (Basel). 2025 Sep 12;15(9):603. doi: 10.3390/bios15090603.

DOI:10.3390/bios15090603
PMID:41002342
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12467597/
Abstract

Matrix metalloproteinases (MMPs) are a class of extracellular Zn peptidases involved in various physiological and pathological processes. These enzymes serve as excellent biomarkers for diagnosing various diseases, including cancer and periodontitis, to name a few. MMP levels also serve as a prognostic marker, which helps determine how much the disease has progressed. However, the current methods used to detect MMPs need a large sample volume, carry a high cost, and are not widely accessible to the public due to these challenges. Biosensing techniques tackle these problems by providing an efficient, cost-effective sensor with great sensitivity. This review provides a comprehensive overview of the latest developments and advancements in detecting MMPs using biosensors that employ various detection mechanisms such as electrochemical, colorimetric, and fluorescence methods. Furthermore, we have discussed the challenges and prospects of using MMPs as diagnostic tools.

摘要

基质金属蛋白酶（MMPs）是一类参与多种生理和病理过程的细胞外锌肽酶。这些酶是诊断各种疾病（包括癌症和牙周炎等）的优秀生物标志物。MMP水平也可作为预后标志物，有助于确定疾病的进展程度。然而，目前用于检测MMPs的方法需要大量样本、成本高昂，并且由于这些挑战而无法被公众广泛使用。生物传感技术通过提供一种高效、经济高效且灵敏度高的传感器来解决这些问题。本综述全面概述了使用生物传感器检测MMPs的最新进展，这些生物传感器采用了电化学、比色法和荧光法等各种检测机制。此外，我们还讨论了将MMPs用作诊断工具的挑战和前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d3d/12467597/3fda35d74985/biosensors-15-00603-g011.jpg
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