Forte Valentina, Lucchetti Sabrina, Ciolfi Andrea, Felici Barbara, Possenti Marco, D'Orso Fabio, Morelli Giorgio, Baima Simona
Council for Agricultural Research and Economics, Research Centre for Genomics and Bioinformatics, Via Ardeatina 546, 00178 Rome, Italy.
Council for Agricultural Research and Economics, Research Centre for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy.
Cells. 2025 Sep 17;14(18):1456. doi: 10.3390/cells14181456.
ATHB1, an homeodomain-leucine zipper (HD-Zip) transcription factor, is involved in the control of leaf development and hypocotyl elongation under short-day conditions. As growth adaptation to environmental conditions is essential for plant resilience, we investigated the role of in the interaction between transcriptional regulatory networks and hormone signaling pathways. We found that wounding, flooding and ethylene induce expression. In addition, we found that the ethylene signal transduction pathway is also involved in an age-dependent expression increase in leaves. Conversely, methyl jasmonate (MeJA) application decreases the transcript level. By exploiting mutant and over-expressing (OE) lines, we also found that the ATHB1 level influences plant sensitivity to the inhibitory effect of MeJA treatment on growth. To gain deeper insights into the regulatory pathways affected by , we performed a microarray analysis comparing the transcriptome of wild-type and mutant plants following exposure to MeJA. Remarkably, although the response to the MeJA treatment was not impaired in , several genes involved in jasmonate and salycilic acid signaling were already downregulated in seedlings under normal conditions compared to the wild type. Thus, our study suggests that may integrate different hormone signaling pathways to influence plant growth under various stress conditions.
ATHB1是一种同源异型域-亮氨酸拉链（HD-Zip）转录因子，参与短日条件下叶片发育和下胚轴伸长的调控。由于生长对环境条件的适应对植物的恢复力至关重要，我们研究了ATHB1在转录调控网络与激素信号通路相互作用中的作用。我们发现，伤口、水淹和乙烯会诱导ATHB1表达。此外，我们还发现乙烯信号转导途径也参与叶片中随年龄增长的ATHB1表达增加。相反，施用茉莉酸甲酯（MeJA）会降低ATHB1转录水平。通过利用突变体和过表达（OE）株系，我们还发现ATHB1水平影响植物对MeJA处理对生长的抑制作用的敏感性。为了更深入地了解受ATHB1影响的调控途径，我们进行了微阵列分析，比较了野生型和ATHB1突变体植株在暴露于MeJA后的转录组。值得注意的是，尽管ATHB1突变体对MeJA处理的反应没有受损，但与野生型相比，在正常条件下，一些参与茉莉酸和水杨酸信号传导的基因在ATHB1突变体幼苗中已经下调。因此，我们的研究表明，ATHB1可能整合不同的激素信号通路，以在各种胁迫条件下影响植物生长。
