Jung Eun Kyung, Salam S M Abdus, Jang Hye-Bin, Koo Joo Yeon, Jahan Eshrat, Kim Sun-Ae, Lee Ji Young, Lee Kyung-Hwa, Yoon Tae Mi
Department of Otolaryngology-Head and Neck Surgery, Chonnam National University Medical School and Hwasun Hospital, Gwangju 58128, Republic of Korea.
Department of Pathology, Chonnam National University Medical School and Hwasun Hospital, Gwangju 58128, Republic of Korea.
Curr Oncol. 2025 Sep 17;32(9):520. doi: 10.3390/curroncol32090520.
The survival rate of patients with advanced laryngeal cancer has not substantially improved over time. RNA sequencing analysis identified Keratin-1 (KRT1) as a gene potentially associated with cancer recurrence. This study investigated the association between KRT1 expression and recurrence in advanced laryngeal cancer. RNA sequencing was performed to identify candidate genes associated with recurrence. The effects of KRT1 expression on clinical outcomes were evaluated in patients with laryngeal cancer. Multiple experimental techniques were utilized. RNA sequencing of patient samples demonstrated higher KRT1 gene expression in the recurrence group than in non-recurrent cases. Patients with KRT1-positive immunostaining exhibited trends of worse overall survival (OS) and recurrence-free survival (RFS). In vitro studies showed that KRT1 knockdown suppressed tumor cell invasion, cell migration, and expression of epithelial-mesenchymal transition (EMT)-related genes in human head and neck squamous cell carcinoma (HNSCC) cell lines. KRT1 knockdown enhanced tumor cell apoptosis and exhibited synergistic effects with conventional radiation and chemotherapy treatments. KRT1 may serve as a biomarker for predicting advanced laryngeal cancer recurrence and assist with selecting patients to receive concurrent chemoradiotherapy (CCRT). Further molecular investigations are warranted to determine its effects, but KRT1 has potential as a therapeutic target.
晚期喉癌患者的生存率长期以来并未得到显著改善。RNA测序分析确定角蛋白-1（KRT1）为一个可能与癌症复发相关的基因。本研究调查了KRT1表达与晚期喉癌复发之间的关联。进行RNA测序以鉴定与复发相关的候选基因。在喉癌患者中评估KRT1表达对临床结局的影响。采用了多种实验技术。患者样本的RNA测序显示，复发组中的KRT1基因表达高于未复发病例。KRT1免疫染色呈阳性的患者表现出总生存期（OS）和无复发生存期（RFS）较差的趋势。体外研究表明，在人头颈鳞状细胞癌（HNSCC）细胞系中，敲低KRT1可抑制肿瘤细胞侵袭、细胞迁移以及上皮-间质转化（EMT）相关基因的表达。敲低KRT1可增强肿瘤细胞凋亡，并与传统放疗和化疗治疗产生协同作用。KRT1可能作为预测晚期喉癌复发的生物标志物，并有助于选择接受同步放化疗（CCRT）的患者。有必要进行进一步的分子研究以确定其作用，但KRT1有作为治疗靶点的潜力。
