Zhu Shaoxiong, Wang Ping, Yin Ming, Yin Ying, Yang Guogen, Huang Bo
Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, 230036, Hefei, China.
School of Biological Engineering, Huainan Normal University, 232038, Huainan, China.
Arch Virol. 2025 Sep 26;170(10):213. doi: 10.1007/s00705-025-06413-3.
Here, we present the identification and complete genome sequences of two novel double-stranded RNA (dsRNA) viruses coinfecting the basal fungus Conidiobolus lii isolate RCEF7535. The viruses, named Conidiobolus lii ootivirus 1 (ClOV1) and Conidiobolus lii totivirus 1 (ClTV1), exhibit distinct genomic characteristics. The genome of ClOV1 is 5,909 nucleotides in length and contains two open reading frames (ORFs). ORF1 encodes a 724-amino-acid capsid protein (CP) with a molecular weight of 80.80 kDa that shows 25.11% sequence identity to the CP of Conidiobolus lamprauges totivirus 1. ORF2 encodes a 743-amino-acid RNA-dependent RNA polymerase (RdRp) with a molecular weight of 83.22 kDa that shares 42.03% sequence identity with the RdRp of Conidiobolus chlamydosporus totivirus 2. The genome of ClTV1 is 4,653 nucleotides in length and contains two ORFs, with ORF1 encoding a 690-amino-acid hypothetical protein (HP, 78.18 kDa) and ORF2 encoding a 799-amino-acid RdRp (90.95 kDa). Both ORFs of ClTV1 display high sequence similarity to hypothetical proteins of Wuhan insect virus 27, with 70.25% identity in the HP and 68.76% identity in the RdRp. Phylogenetic analysis based on RdRp sequences showed that ClOV1 grouped with viruses of the genus Ootivirus within the family Ootiviridae, while ClTV1 clustered with viruses of the genus Totivirus of the family Orthototiviridae. This discovery enhances our understanding of mycovirus diversity, particularly in basal fungi, by identifying novel viruses infecting members of the genus Conidiobolus.
在此，我们展示了两种新型双链RNA（dsRNA）病毒的鉴定结果及其完整基因组序列，这两种病毒共同感染了基部真菌鲁氏虫霉分离株RCEF7535。这两种病毒分别命名为鲁氏虫霉卵病毒1（ClOV1）和鲁氏虫霉全病毒1（ClTV1），具有不同的基因组特征。ClOV1的基因组长度为5909个核苷酸，包含两个开放阅读框（ORF）。ORF1编码一个724个氨基酸的衣壳蛋白（CP），分子量为80.80 kDa，与兰氏虫霉全病毒1的CP具有25.11%的序列同一性。ORF2编码一个743个氨基酸的RNA依赖RNA聚合酶（RdRp），分子量为83.22 kDa，与产孢虫霉全病毒2的RdRp具有42.03%的序列同一性。ClTV1的基因组长度为4653个核苷酸，包含两个ORF，其中ORF1编码一个690个氨基酸的假定蛋白（HP，78.18 kDa），ORF2编码一个799个氨基酸的RdRp（90.95 kDa）。ClTV1的两个ORF与武汉昆虫病毒27的假定蛋白均显示出高度序列相似性，HP的同一性为70.25%，RdRp的同一性为68.76%。基于RdRp序列的系统发育分析表明，ClOV1与卵病毒科卵病毒属的病毒聚为一类，而ClTV1与正全病毒科全病毒属的病毒聚为一类。这一发现通过鉴定感染虫霉属成员的新型病毒，增强了我们对真菌病毒多样性的理解，尤其是对基部真菌的理解。
