Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, School of Plant Protection, Anhui Agricultural University, Hefei, 230036, China.
Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei, 230031, China.
Arch Virol. 2020 Aug;165(8):1919-1923. doi: 10.1007/s00705-020-04692-6. Epub 2020 Jun 5.
Mycoviruses are widely distributed in a variety of fungal species. However, few viruses have been reported in basal fungi. A novel non-segmented dsRNA virus was isolated from the basal fungus Conidiobolus sp. of the phylum Zoopagomycota, which has been named "Conidiobolus non-segmented RNA virus 1" (CNRV1). The complete genome sequence of CNRV1 was determined by dsRNA extraction, next-generation sequencing, and RACE. The genome of CNRV1 dsRNA is 3,092 bp in length and contains two open reading frames (ORFs) predicted to encode a subgenomic protein 1 (sgP1) and a putative RNA-dependent RNA polymerase (RdRp). Multiple sequence alignment and phylogenetic analysis based on RdRp sequences from selected dsRNA viruses showed that CNRV1 shared 31.9% sequence identity with Nigrospora oryzae unassigned RNA virus 1 (NoNRV1) and clustered with NoNRV1 and four other mycoviruses. These viruses are unassigned and distant from members of the family Partitiviridae, although they were previously considered partitivirus-like viruses. Thus, CNRV1 is a novel member of proposed genus "Unirnavirus", and is the first dsRNA sequence reported from a member of the phylum Zoopagomycota. This study extends our knowledge about mycoviruses in basal fungi.
真菌病毒广泛分布于各种真菌物种中。然而，在基础真菌中报道的病毒很少。一种新型的非分段双链 RNA 病毒从基础真菌动孢菌（Zoopagomycota 门）中分离出来，被命名为“Conidiobolus 非分段 RNA 病毒 1”（CNRV1）。通过 dsRNA 提取、下一代测序和 RACE 确定了 CNRV1 的完整基因组序列。CNRV1 的 dsRNA 基因组长 3092bp，包含两个开放阅读框（ORF），预测分别编码亚基因组蛋白 1（sgP1）和推定的 RNA 依赖性 RNA 聚合酶（RdRp）。基于 RdRp 序列从选定的 dsRNA 病毒选择的序列比对和系统发育分析表明，CNRV1 与稻黑孢霉未分类 RNA 病毒 1（NoNRV1）的序列同一性为 31.9%，并与 NoNRV1 和其他四种真菌病毒聚类。这些病毒尚未分类，与二分体病毒科的成员距离较远，尽管它们以前被认为是二分体病毒样病毒。因此，CNRV1 是提议的“Unirnavirus”属的一个新成员，也是首次从 Zoopagomycota 门成员中报告的 dsRNA 序列。本研究扩展了我们对基础真菌中真菌病毒的认识。
