Huang Wen Xin, Yang Li E, Huang Yan Bin, Xu Tao Sha, Zhong Jie, Wang Ya Rong, Xiao Yan Song
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, 410128, Changsha City, Hunan Province, P.R. China.
Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, 650205, Kunming City, Yunan Province, P.R. China.
Arch Virol. 2025 Aug 6;170(9):189. doi: 10.1007/s00705-025-06363-w.
In this study, a novel double-stranded RNA (dsRNA) mycovirus was isolated from the phytopathogenic fungus Fusarium commune, the causal agent of tobacco root rot, and designated "Fusarium commune chrysovirus 1" (FcCV1). The complete genome of FcCV1 consists of five dsRNA fragments with lengths of 3670 bp, 3242 bp, 2866 bp, 2829 bp, and 1258 bp, designated as dsRNA1 to dsRNA5 according to their size. Each of these five dsRNA segments contains a single open reading frame (ORF), designated ORF1 to ORF5, with strictly conserved termini in their 5' and 3' untranslated regions. Notably, ORF1 encodes a potential RNA-dependent RNA polymerase (RdRp) comprising 1151 amino acids (aa), which exhibits the highest sequence identity (56.14%) with the RdRp of Tolypocladium cylindrosporum virus 2 (TcV2). ORF2, ORF3, and ORF5 encode hypothetical proteins of unknown function, displaying amino acid sequence similarity to proteins encoded by different dsRNAs of chrysoviruses. ORF4 encodes a predicted coat protein (CP). Phylogenetic analysis based on RdRp sequences placed FcCV1 within the genus Betachrysovirus of the family Chrysoviridae. This is the first report of a novel chrysovirus infecting F. commune.
在本研究中，从引起烟草根腐病的植物病原真菌尖孢镰刀菌中分离出一种新型双链RNA（dsRNA）真菌病毒，并将其命名为“尖孢镰刀菌金黄病毒1”（FcCV1）。FcCV1的完整基因组由五个dsRNA片段组成，长度分别为3670 bp、3242 bp、2866 bp、2829 bp和1258 bp，根据大小分别命名为dsRNA1至dsRNA5。这五个dsRNA片段中的每一个都包含一个单一的开放阅读框（ORF），分别命名为ORF1至ORF5，其5'和3'非翻译区具有严格保守的末端。值得注意的是，ORF1编码一种潜在的RNA依赖性RNA聚合酶（RdRp），由1151个氨基酸（aa）组成，与柱孢绿僵菌病毒2（TcV2）的RdRp具有最高的序列同一性（56.14%）。ORF2、ORF3和ORF5编码功能未知的假定蛋白，与金黄病毒不同dsRNA编码的蛋白具有氨基酸序列相似性。ORF4编码一种预测的衣壳蛋白（CP）。基于RdRp序列的系统发育分析将FcCV1置于金黄病毒科β金黄病毒属内。这是关于一种感染尖孢镰刀菌的新型金黄病毒的首次报道。
