Zalazar David, Feng Jiayi, Banyard Derek A, Aliaghaei Marzieh, Widgerow Alan D, Haun Jered B
Department of Biomedical Engineering, University of California Irvine, Irvine, CA 92697, USA.
Sayenza Biosciences Inc., Irvine, CA 92617, USA.
Bioengineering (Basel). 2025 Aug 26;12(9):918. doi: 10.3390/bioengineering12090918.
Autologous fat grafting of human lipoaspirate (LA) is increasingly used in reconstructive and cosmetic surgery for lipofilling and stem cell-rich "nanofat" reinjection for regenerative medicine. While commercial devices (e.g., REVOLVE and Puregraft) are available, many surgeons use non-standardized manual washing techniques, leading to inconsistent graft retention (20-80%). Moreover, no system can unite washing directly with mechanical processing to produce a nanofat-like product directly from raw LA. We developed a novel preparation device (PD) that is designed for peristaltic pump-driven washing of LA and can be seamlessly combined with our previously developed Emulsification and Micronization Device (EMD) into an automated closed-loop platform. Human LA samples were washed with the PD and compared to standard manual washing via visual colorimetric analysis. We then evaluated the mechanical processing of PD-washed LA using our EMD and assessed cell count, viability, and stromal vascular fraction-derived subpopulations (i.e., mesenchymal stem cells, endothelial progenitor cells (EPCs), pericytes, transit-amplifying (TA) progenitor cells, and supra-adventitial adipose stromal cells). Recirculating LA through the PD for at least one minute resulted in sufficient mixing, producing LA with equivalent color and quality to manual washing. Integrating the EMD within a platform enabled both washing and mechanical processing under peristaltic flow, enriching key subpopulations compared to manual methods. Thus, our fluidic platform effectively washes LA in a closed-loop system, minimizing LA tissue manipulation and opportunity for contamination while also simplifying the workflow for mechanical processing. Further refinement and automation of this platform would enhance the reproducibility and quality of small-volume fat grafts, cell-assisted lipotransfer, and stem/progenitor cell injections to promote wound healing and angiogenesis.
人体抽脂物（LA）的自体脂肪移植越来越多地用于重建和整形手术中的脂肪填充以及再生医学中富含干细胞的“纳米脂肪”再注射。虽然有商用设备（如REVOLVE和Puregraft），但许多外科医生使用非标准化的手工清洗技术，导致移植保留率不一致（20%-80%）。此外，没有系统能将清洗与机械处理直接结合，以直接从原始LA生产出类似纳米脂肪的产品。我们开发了一种新型制备设备（PD），该设备设计用于蠕动泵驱动的LA清洗，并且可以与我们之前开发的乳化和微粉化设备（EMD）无缝结合成一个自动化闭环平台。用PD清洗人体LA样本，并通过视觉比色分析与标准手工清洗进行比较。然后，我们使用EMD评估PD清洗后的LA的机械处理，并评估细胞计数、活力以及基质血管成分衍生的亚群（即间充质干细胞、内皮祖细胞（EPC）、周细胞、过渡扩增（TA）祖细胞和外膜上脂肪基质细胞）。使LA在PD中循环至少一分钟可实现充分混合，产生颜色和质量与手工清洗相当的LA。将EMD集成到一个平台中，能够在蠕动流条件下进行清洗和机械处理，与手工方法相比，可富集关键亚群。因此，我们的流体平台在闭环系统中有效地清洗LA，最大限度地减少LA组织操作和污染机会，同时还简化了机械处理的工作流程。该平台的进一步优化和自动化将提高小体积脂肪移植、细胞辅助脂肪移植和干细胞/祖细胞注射的可重复性和质量，以促进伤口愈合和血管生成。
