Chiarot Alessandra, Minhas Mahek, de Maat Nicoletta M, Doan Jenny, Nilsson Mats I, Hettinga Bart P, Faghih Mehrnoosh, Neal Michael S, Nederveen Joshua P, Tarnopolsky Mark A
Department of Pediatrics, McMaster University Medical Center (MUMC), Hamilton, ON L86 4L8, Canada.
Department of Kinesiology, McMaster University, Hamilton, ON L86 4L8, Canada.
Biomolecules. 2025 Aug 30;15(9):1258. doi: 10.3390/biom15091258.
Ovarian aging is characterized by mitochondrial dysfunction, oxidative stress, and inflammation. The development of adjunctive treatments that mitigate age-related subfertility is warranted. We examined the benefits of nutraceutical supplementation (FE; Fertility Enhancer) with mitochondrial antioxidants, anti-inflammatory agents, metabolic activators, vitamins and minerals, and amino acids on ovarian aging, metabolic activity, and reproductive success in young (Y; 6-month-old) and middle-aged (O; 11-month-old) female C57BL/6J mice. The mice were fed calorie- and macronutrient-matched diets w/wo the FE supplement for three months and harem mated twice. Daily FE supplementation promoted significant body re-composition, including loss of white adipose tissue (gWAT: -36% vs. CON, < 0.001), gain of skeletal muscle (SkM: +67% vs. CON, < 0.001), and improved SkM/gWAT ratio (+185% vs. CON, < 0.001). Metabolic testing showed enhanced fat oxidation (+38%, < 0.01) and energy expenditure (+7%, = 0.051) in FE mice. Breeding and immunoblotting data demonstrated improved reproductive success (Y-CON: 44%, Y-FE: 89%, O-CON: 0%, O-FE: 18%) and a modest attenuation of ovarian aging markers in both FE groups. We surmise that a multi-ingredient supplement, such as the Fertility Enhancer, may improve body re-composition, metabolic activity, and markers of ovarian aging, thus enhancing reproductive health and fertility in females.
卵巢衰老的特征是线粒体功能障碍、氧化应激和炎症。开发减轻与年龄相关的生育力低下的辅助治疗方法是必要的。我们研究了营养补充剂（FE；生育增强剂），其含有线粒体抗氧化剂、抗炎剂、代谢激活剂、维生素、矿物质和氨基酸，对年轻（Y；6个月大）和中年（O；11个月大）雌性C57BL/6J小鼠卵巢衰老、代谢活性和生殖成功率的影响。给小鼠喂食含或不含FE补充剂的热量和常量营养素匹配的饮食三个月，并进行两次后宫式交配。每日补充FE促进了显著的身体成分重新组合，包括白色脂肪组织减少（腹股沟白色脂肪组织：-36% vs. CON，<0.001）、骨骼肌增加（SkM：+67% vs. CON，<0.001）以及SkM/腹股沟白色脂肪组织比率改善（+185% vs. CON，<0.001）。代谢测试显示FE小鼠的脂肪氧化增强（+38%，<0.01）和能量消耗增加（+7%，P = 0.051）。繁殖和免疫印迹数据表明，两个FE组的生殖成功率均有所提高（Y-CON组：44%，Y-FE组：89%，O-CON组：0%，O-FE组：18%），并且卵巢衰老标志物有适度减轻。我们推测，像生育增强剂这样的多成分补充剂可能会改善身体成分重新组合、代谢活性和卵巢衰老标志物，从而增强雌性的生殖健康和生育力。
