Smirnova Evgeniya V, Barsova Ekaterina V, Varlamov Dmitriy A, Kramarov Vladimir M, Blagodatskikh Konstantin A, Ignatov Konstantin B
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.
Institute of Translational Medicine, Pirogov Russian National Research Medical University, 117513 Moscow, Russia.
Biomolecules. 2025 Sep 12;15(9):1313. doi: 10.3390/biom15091313.
Nucleic acid amplification methods are widely used in science, medicine and forensics for molecular biological assays and for the detection of genetic material. The newly developed strand displacement chain reaction (SDCR) method is a hybrid amplification technique based on polymerase chain reaction (PCR) and isothermal nucleic acid amplification. Here, we compared conventional PCR, the "gold standard" for molecular diagnostic assays, with the SDCR method by performing real-time amplification assays using human, bacterial and viral genetic materials. In the assays, SDCR demonstrated very high sensitivity and amplification efficiency. We found that the SDCR method provided an amplification factor above three, which noticeably outperformed that of PCR amplification and enabled a marked reduction in the number of cycles in comparison with PCR. Therefore, the new hybrid amplification technique could be extremely useful for the detection of genetic material and the development of new diagnostic kits.
核酸扩增方法广泛应用于科学、医学和法医学领域，用于分子生物学检测和遗传物质的检测。新开发的链置换链式反应（SDCR）方法是一种基于聚合酶链式反应（PCR）和等温核酸扩增的杂交扩增技术。在此，我们通过使用人类、细菌和病毒遗传物质进行实时扩增检测，将分子诊断检测的“金标准”——传统PCR与SDCR方法进行了比较。在检测中，SDCR显示出非常高的灵敏度和扩增效率。我们发现，SDCR方法提供了超过三倍的扩增倍数，明显优于PCR扩增，并且与PCR相比能够显著减少循环次数。因此，这种新的杂交扩增技术对于遗传物质的检测和新型诊断试剂盒的开发可能极其有用。
