Jarisarapurin Wattanased, Puksasook Thanchanok, Kumphune Sarawut, Chaiya Nattanicha, Pongwan Pawinee, Pholsin Rawisada, Sramala Issara, Tapaneeyakorn Satita
National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Khlong Luang District, Pathum Thani 12120, Thailand.
Biomedical Engineering Institute, CMU-BIOPOLIS Building, Chiang Mai University, Mae-Hia District, Chiang Mai 50100, Thailand.
Antioxidants (Basel). 2025 Aug 24;14(9):1043. doi: 10.3390/antiox14091043.
Repeated UV exposure, air pollution, and toxins promote skin oxidative stress. ROS destroy macromolecules, changing cellular mechanisms and signaling cascades. Inflammation and injury to skin cells degrade function and accelerate aging, causing wrinkles, firmness loss, and dermatological disorders. (GI) contains phytochemical antioxidants such polyphenols and triterpenoids that lower ROS and strengthen skin. GI extracts (GIEs) have never been examined for their effects on dermal skin fibroblasts' oxidative stress and intracellular cytoprotective mechanisms. In this study, GIEs were prepared as a water extract (GIE0) and ethanol extracts with concentrations ranging from 20% to 95% / (GIE20, GIE40, GIE60, GIE80, and GIE95). These extracts were assessed for phytochemical content, antioxidant capacity, and free radical scavenging efficacy. The results were compared to a commercially available native Gymnema extract (NGE) obtained from . During principal component analysis (PCA), the most effective extracts were identified and subsequently evaluated for their ability to mitigate oxidative stress in fibroblasts. Cytoprotective effects of GIE and NGE against HO-induced human dermal fibroblast injury were investigated by cell viability, intracellular ROS production, and signaling pathways. GIE0, GIE80, GIE95, and NGE were the best antioxidants. By preserving ROS balance and redox homeostasis, GIE and NGE reduce fibroblast inflammation and oxidative stress-induced damage. Decreased ROS levels reduce MAPK/AP-1/NF-κB and PI3K/AKT/NF-κB signaling pathways, diminishing inflammatory cytokines. In conclusion, GIE and NGE have antioxidant and anti-inflammatory capabilities that can reduce HO-induced fibroblast oxidative stress and damage, thereby preventing skin aging and targeting cancer-associated fibroblasts.
反复暴露于紫外线、空气污染和毒素会加剧皮肤氧化应激。活性氧（ROS）会破坏大分子，改变细胞机制和信号级联反应。皮肤细胞的炎症和损伤会降低其功能并加速衰老，导致皱纹、皮肤松弛和皮肤病。匙羹藤（GI）含有多酚和三萜类等植物化学抗氧化剂，可降低ROS水平并增强皮肤健康。此前从未研究过匙羹藤提取物（GIEs）对皮肤成纤维细胞氧化应激和细胞内细胞保护机制的影响。在本研究中，制备了水提取物（GIE0）和浓度范围为20%至95%的乙醇提取物（GIE20、GIE40、GIE60、GIE80和GIE95）。对这些提取物的植物化学成分、抗氧化能力和自由基清除功效进行了评估。将结果与从……获得的市售天然匙羹藤提取物（NGE）进行比较。在主成分分析（PCA）过程中，确定了最有效的提取物，随后评估了它们减轻成纤维细胞氧化应激的能力。通过细胞活力、细胞内ROS产生和信号通路研究了GIE和NGE对过氧化氢（HO）诱导的人皮肤成纤维细胞损伤的细胞保护作用。GIE0、GIE80、GIE95和NGE是最佳抗氧化剂。通过维持ROS平衡和氧化还原稳态，GIE和NGE可减轻成纤维细胞炎症和氧化应激诱导的损伤。ROS水平降低会减少丝裂原活化蛋白激酶/活化蛋白-1/核因子-κB（MAPK/AP-1/NF-κB）和磷脂酰肌醇-3激酶/蛋白激酶B/核因子-κB（PI3K/AKT/NF-κB）信号通路，减少炎性细胞因子。总之，GIE和NGE具有抗氧化和抗炎能力，可减少HO诱导的成纤维细胞氧化应激和损伤，从而预防皮肤衰老并靶向癌症相关成纤维细胞。
