一项关于Walt.果实的研究：评估其在结直肠腺癌细胞中的抗氧化、抗炎和抗癌活性及其植物化学特征
A Study of the Fruits of Walt.: Evaluation of the Antioxidant, Anti-Inflammatory, and Anti-Cancer Activities in Colorectal Adenocarcinoma Cells in Relation to Phytochemical Profile.
作者信息
Bernardi Clizia, Gaslonde Thomas, Finetti Federica, Benmaouche Salim, Macrì Giulia, Dugay Annabelle, Cuyamendous Claire, Bouzidi Chouaha, Loizzo Monica Rosa, Belmont Philippe, Tundis Rosa, Trabalzini Lorenza, Deguin Brigitte
机构信息
Department of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy.
Faculté de Pharmacie de Paris, Université Paris Cité, U.M.R. CiTCoM (n°8038-CNRS/Université Paris Cité), F-75006 Paris, France.
出版信息
Antioxidants (Basel). 2025 Sep 14;14(9):1116. doi: 10.3390/antiox14091116.
The chemical profiles and potential anti-inflammatory, antioxidant, and anticancer activities of the aqueous extract and fractions of fresh fruits were studied. Iridoids, flavonoids, and phenolic compounds represent the main phytochemical classes. Nine of the ten iridoids detected are acyl-iridoids. Significant amounts of catalpol and catalposide were found. The antioxidant activity of iridoids was demonstrated by HPTLC analysis coupled with a DPPH derivatization and by applying four in vitro tests, such as DPPH, ABTS, FRAP, and the β-carotene bleaching test. extract and fractions were also evaluated for their anti-cancer activity using in vitro models of colorectal cancer (HT29 and HCT166 cell lines), and focusing on the effect of the different fractions on inflammation and oxidative stress, key factors that drive the onset and progression of colon cancer.
对新鲜水果的水提取物及其馏分的化学特征以及潜在的抗炎、抗氧化和抗癌活性进行了研究。环烯醚萜类、黄酮类和酚类化合物是主要的植物化学类别。检测到的十种环烯醚萜类中有九种是酰基环烯醚萜类。发现了大量的梓醇和梓苷。通过HPTLC分析结合DPPH衍生化以及应用四种体外试验（如DPPH、ABTS、FRAP和β-胡萝卜素漂白试验）证明了环烯醚萜类的抗氧化活性。还使用结肠直肠癌的体外模型（HT29和HCT166细胞系）评估了提取物及其馏分的抗癌活性，并重点关注不同馏分对炎症和氧化应激的影响，炎症和氧化应激是驱动结肠癌发生和发展的关键因素。
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