Fondevila Guillermo, Fatmi Habib, Fernando Pilar, Dapoza Carlos, Fondevila Manuel
Departamento de Producción Animal y Ciencia de los Alimentos, Instituto Agroalimentario de Aragón (IA2), Universidad de Zaragoza-CITA, 50013 Zaragoza, Spain.
Evonik Operations GmbH, 08021 Barcelona, Spain.
Insects. 2025 Sep 22;16(9):989. doi: 10.3390/insects16090989.
The effects of Lys and Ile supplementation in a barley-based substrate on growth performance and nutrient deposition in larvae were evaluated. Six trays per treatment containing sixty larvae were assigned to five treatments in two 21-day periods: barley (B); an 85% barley and 15% soybean meal mixture (BS) and B supplemented with synthetic Lys (BL), Ile (BI) or a combination of both (BLI), as potential limiting amino acids (AA) for larval growth. Supplementing AAs was provided in agar cubes twice per week. Larval performance was measured, and larvae were analyzed for nutrient content and AA profile. Final larval weight increased ( = 0.042) in BS and BL compared to B, with intermediate values for BI and BLI. Larval contents (mg/larva) in total AAs and seven key AAs (sum of Lys, Met, Cys, Thr, Trp, Val and Ile) were higher in BS-fed larvae than in BL and BLI, with the lowest values for BI and B ( < 0.001). Barley supplementation with Lys improved larval AA content, although the levels remained lower than those observed with BS. Conversely, Ile supplementation had no apparent effect on overall AA deposition compared to Lys alone, suggesting the presence of additional limiting AAs for optimal larval growth.
评估了在以大麦为基础的底物中添加赖氨酸（Lys）和异亮氨酸（Ile）对幼虫生长性能和营养物质沉积的影响。在两个21天的时间段内，将每个处理的六个托盘（每个托盘包含60只幼虫）分配到五种处理中：大麦（B）；85%大麦和15%豆粕的混合物（BS），以及添加了合成赖氨酸（BL）、异亮氨酸（BI）或两者组合（BLI）的B，作为幼虫生长的潜在限制性氨基酸（AA）。每周两次在琼脂块中提供氨基酸补充剂。测量幼虫性能，并分析幼虫的营养成分和氨基酸谱。与B相比，BS和BL中的最终幼虫体重增加（P = 0.042），BI和BLI的值居中。BS喂养的幼虫中总氨基酸和七种关键氨基酸（赖氨酸、蛋氨酸、半胱氨酸、苏氨酸、色氨酸、缬氨酸和异亮氨酸的总和）的幼虫含量（mg/幼虫）高于BL和BLI，BI和B中的值最低（P < 0.001）。添加赖氨酸的大麦提高了幼虫的氨基酸含量，尽管其水平仍低于BS中的水平。相反，与单独添加赖氨酸相比，添加异亮氨酸对整体氨基酸沉积没有明显影响，这表明存在其他限制幼虫最佳生长的氨基酸。
