Yang Yuting, Tan Shunyang, Wang Qinli, Wang Fu, Zhang Youjun
Ministry of Agriculture and Rural Affairs Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), Hubei Engineering Technology Center for Pest Forewarning and Management, Institute of Insect Sciences, Yangtze University, Jingzhou, Hubei 434000, China.
Ministry of Agriculture and Rural Affairs Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), Hubei Engineering Technology Center for Pest Forewarning and Management, Institute of Insect Sciences, Yangtze University, Jingzhou, Hubei 434000, China.
Int J Biol Macromol. 2025 Jan;284(Pt 2):138179. doi: 10.1016/j.ijbiomac.2024.138179. Epub 2024 Nov 28.
Bradysia odoriphaga (Diptera: Sciaridae) is a devastating underground pest that can cause serious economic losses. Odorant binding proteins (OBPs) are crucial components of the insect olfactory system, playing key roles in locating host plants, oviposition sites, and mates. Therefore, they are considered potential targets for pest control. Here, we obtained one OBP gene (BodoOBP7) from the antennal transcriptome of B. odoriphaga, and observed that the expression level of BodoOBP7 was primarily in the antennae of both sexes, with significantly higher expression level in females than in males. Fluorescence competitive binding assays indicated that BodoOBP7 exhibited strong binding affinities for the six host plant volatiles, including propyl disulfide, dipropyl trisulfide, dimethyl trisulfide, 2-tridecanone, 2-undecanone and alpha-ionone. Subsequently, homology modeling, molecular docking and site-directed mutagenesis revealed that four key amino acid residues (Phe79, Phe99, Ile96, Leu100) participate in the binding of BodoOBP7 with six host plant volatiles. Our results demonstrate that BodoOBP7 is involved in olfactory recognition in B. odoriphaga. These findings may enhance our understanding of the interaction mechanisms between host plants and B. odoriphaga, potentially offering new perspectives for the development of effective green control strategies.
韭菜迟眼蕈蚊（双翅目：眼蕈蚊科）是一种极具破坏性的地下害虫，会造成严重的经济损失。气味结合蛋白（OBPs）是昆虫嗅觉系统的关键组成部分，在定位寄主植物、产卵场所和配偶方面发挥着关键作用。因此，它们被认为是害虫防治的潜在靶点。在此，我们从韭菜迟眼蕈蚊的触角转录组中获得了一个OBP基因（BodoOBP7），并观察到BodoOBP7的表达水平主要在雌雄两性的触角中，雌性的表达水平显著高于雄性。荧光竞争结合试验表明，BodoOBP7对六种寄主植物挥发物表现出强烈的结合亲和力，包括丙基二硫化物、二丙基三硫化物、二甲基三硫化物、2-十三酮、2-十一酮和α-紫罗兰酮。随后，同源建模、分子对接和定点诱变表明，四个关键氨基酸残基（Phe79、Phe99、Ile96、Leu100）参与了BodoOBP7与六种寄主植物挥发物的结合。我们的结果表明，BodoOBP7参与了韭菜迟眼蕈蚊的嗅觉识别。这些发现可能会增进我们对寄主植物与韭菜迟眼蕈蚊之间相互作用机制的理解，为开发有效的绿色防治策略提供新的视角。
