Tsubokawa Masaya, Shimizu Yoshiki, Yazaki Misato, Shimodan Shieri, Noguchi Masayuki, Yamazaki Arisa, Watanabe Tomomichi, Ocho Makoto, Sakurada Tsuyoshi, Hirose Yoshie, Saito Jiro, Ishii Yuri
FANCL Research Institute, Yokohama 244-0806, Japan.
Ginza Yoshie Clinic, Yukeikai Medical Corporation, V88 Building 5F, 2-5-11 Ginza, Chuo-ku, Tokyo 104-0061, Japan.
Int J Mol Sci. 2025 Sep 10;26(18):8799. doi: 10.3390/ijms26188799.
Recently, senescent T cells in the peripheral blood have been detected using senescence-associated β-galactosidase (SA-βGal) activity and have been used as an endpoint in clinical trials. However, the epidemiological association between the abundance of SA-βGal-stained senescent CD8+ T cells and chronological age has not been fully elucidated. To examine the correlation between the proportion of SA-βGal CD8+ T cells and age, we analyzed previously collected clinical trial data. We conducted a cross-sectional analysis of 632 Japanese adults aged 40-59 years who participated in the screening phase of a clinical trial. To characterize senescent CD8+ T cells, we measured the proportion of SA-βGal in total CD8+ T cells and each subset-naïve, central memory (TCM), effector memory (TEM), and terminally differentiated effector memory (TEMRA). We then calculated the correlation coefficients between the proportion of SA-βGal CD8+ T cells and age. The proportion of SA-βGal cells in total CD8+ T cells, naïve, TCM, TEM, and TEMRA CD8+ T cells increased significantly with age. In Japanese adults, the proportion of SA-βGal in CD8+ T cells may serve as a useful biomarker of immune senescence.
最近，外周血中的衰老T细胞已通过衰老相关β-半乳糖苷酶（SA-βGal）活性检测到，并已在临床试验中用作终点指标。然而，SA-βGal染色的衰老CD8 + T细胞丰度与实际年龄之间的流行病学关联尚未完全阐明。为了研究SA-βGal CD8 + T细胞比例与年龄之间的相关性，我们分析了先前收集的临床试验数据。我们对632名年龄在40 - 59岁之间参与一项临床试验筛查阶段的日本成年人进行了横断面分析。为了表征衰老的CD8 + T细胞，我们测量了总CD8 + T细胞以及每个亚群（初始、中枢记忆（TCM）、效应记忆（TEM）和终末分化效应记忆（TEMRA））中SA-βGal的比例。然后我们计算了SA-βGal CD8 + T细胞比例与年龄之间的相关系数。总CD8 + T细胞、初始、TCM、TEM和TEMRA CD8 + T细胞中SA-βGal细胞的比例随年龄显著增加。在日本成年人中，CD8 + T细胞中SA-βGal的比例可能作为免疫衰老的一个有用生物标志物。
