Muravenko Olga V, Amosova Alexandra V, Semenov Alexey R, Kalnyuk Julia V, Khazieva Firdaus M, Korotkikh Irina N, Basalaeva Irina V, Badaeva Ekaterina D, Zoshchuk Svyatoslav A, Yurkevich Olga Yu
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov St., 119991 Moscow, Russia.
All-Russian Institute of Medicinal and Aromatic Plants, Federal Agency for Scientific Organizations, 113628 Moscow, Russia.
Int J Mol Sci. 2025 Sep 22;26(18):9240. doi: 10.3390/ijms26189240.
L. (Polemoniaceae) is a perennial flowering plant native to Eurasia and North America, which is used as a fodder, medicinal, and ornamental plant. Many issues related to the taxonomy and origin of this valuable species still remain unclear. The intraspecific genetic variability of and chromosomal organization of its genome are insufficiently studied. For the first time, we analyzed NGS genomic data of using ReapeatExplorer2/TAREAN/DANTE Pipelines. In its repeatome, we identified 66.08% of Class I retrotransposons; 0.57% of Class II transposons; 0.42% of ribosomal DNA; and 0.87% of satellite DNA (six high-confident and three low-confident putative satellite DNAs). FISH chromosome mapping of seven tandem DNAs was carried out in two varieties and two wild populations. Our results demonstrated the effectiveness of using satDNAs Pol_C 46 and Pol_C 33 in combination with 45S rDNA and 5S rDNA for precise chromosome identification. This approach allowed us to study intraspecific chromosomal variability and detect chromosomal rearrangements in the studied accessions of , which could be related to the speciation process. These novel molecular markers are important for chromosome studies within to clarify its taxonomy and phylogeny, and also, they expand the potential of different breeding programs.
L.（花荵科）是一种多年生开花植物，原产于欧亚大陆和北美洲，用作饲料、药用和观赏植物。关于这个珍贵物种的分类学和起源的许多问题仍然不清楚。其种内遗传变异性和基因组的染色体组织研究不足。我们首次使用ReapeatExplorer2/TAREAN/DANTE管道分析了L.的NGS基因组数据。在其重复基因组中，我们鉴定出66.08%的I类反转录转座子；0.57%的II类转座子；0.42%的核糖体DNA；以及0.87%的卫星DNA（六个高可信度和三个低可信度的假定卫星DNA）。在两个L.品种和两个野生种群中对七个串联DNA进行了荧光原位杂交染色体定位。我们的结果证明了使用卫星DNA Pol_C 46和Pol_C 33与45S rDNA和5S rDNA结合进行精确染色体鉴定的有效性。这种方法使我们能够研究L.研究材料中的种内染色体变异性并检测染色体重排，这可能与物种形成过程有关。这些新的分子标记对于L.内的染色体研究以阐明其分类学和系统发育很重要，而且，它们扩展了不同育种计划的潜力。
