Furnica Dan-Tiberiu, Falkenstein Julia, Dittmer Silke, Steinmann Joerg, Rath Peter-Michael, Kirchhoff Lisa
Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, 45122 Essen, Germany.
Institute of Clinical Hygiene, Medical Microbiology and Infectiology, Klinikum Nürnberg, Paracelsus Medical University, 90419 Nuremberg, Germany.
Microorganisms. 2025 Aug 31;13(9):2040. doi: 10.3390/microorganisms13092040.
is an opportunistic filamentous fungus that primarily affects the respiratory tract of the human body. Depending on its host's immune response, the pathogen can cause invasive pulmonary aspergillosis (IPA). Biofilm formation by increases virulence and resistance against antifungals and immune response and is one important factor in IPA development. Here, two human-like models, precision cut lung slices (PCLS) and a biofilm co-culture model, have been developed to test the anti-biofilm activity of voriconazole, amphotericin B, as well as luliconazole against . In both assays, metabolically active biofilms were examined at different biofilm developmental stages using an XTT assay. A decrease in the metabolic activity of the fungal biofilms was detected for each of the tested agents in both assays. Significant anti-biofilm effects exist against early-stage biofilm in the co-culture model. In the PCLS assay, amphotericin B showed the strongest inhibition after 24 h. In conclusion, the applied PCLS ex vivo model can be used to study the property and activity of certain antifungal compounds against biofilm. With its close resemblance to human conditions, the PCLS model has the potential for improving the current understanding of biofilm treatments in laboratory settings.
是一种机会性丝状真菌，主要影响人体呼吸道。根据宿主的免疫反应，该病原体可导致侵袭性肺曲霉病（IPA）。形成生物膜会增加其毒力以及对抗真菌药物和免疫反应的抵抗力，是IPA发展的一个重要因素。在此，已开发出两种类人模型，即精密肺切片（PCLS）和生物膜共培养模型，以测试伏立康唑、两性霉素B以及卢立康唑对的抗生物膜活性。在这两种试验中，使用XTT试验在不同生物膜发育阶段检测代谢活跃的生物膜。在两种试验中，每种受试药物均检测到真菌生物膜代谢活性降低。在共培养模型中，对早期生物膜存在显著的抗生物膜作用。在PCLS试验中，两性霉素B在24小时后显示出最强的抑制作用。总之，所应用的PCLS体外模型可用于研究某些抗真菌化合物对生物膜的特性和活性。由于其与人体状况极为相似，PCLS模型有潜力在实验室环境中增进当前对生物膜治疗的理解。
