Szumska Magdalena, Maciejczyk Maciej, Janoszka Beata, Damasiewicz-Bodzek Aleksandra, Nowak Agnieszka, Tyrpień-Golder Krystyna
Department of Chemistry, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, Poland.
Molecules. 2025 Sep 18;30(18):3787. doi: 10.3390/molecules30183787.
Marshmallows are confectioneries that are popular among children and teenagers around the world. Barbecues and the consumption of grilled marshmallows, especially by children, have become fashionable in many countries. Grilled marshmallows may contain carcinogenic polycyclic aromatic hydrocarbons (PAHs). Hydroxy-PAHs (OH-PAHs) concentration in the urine of volunteers after the consumption of grilled marshmallows, as biomarkers of exposure to PAHs, have been determined. A total of 24 participants consumed marshmallows grilled under similar conditions. Urine samples were collected before and after the consumption of grilled marshmallows. 1-hydroxypyrene and 9-hydroxyphenanthrene concentrations in urine samples were determined using the HPLC-FLD technique after enzymatic hydrolysis and isolation by solid-phase extraction (SPE). The average concentration of 1-hydroxypyrene was 0.21 ± 0.16 µg/g creatinine and of 9-hydroxypenanthrene was 2.78 ± 2.55 µg/g creatinine. The concentrations of OH-PAHs in the urine of volunteers eating colored grilled marshmallows were higher compared to the consumption of white ones. In the case of 9-hydroxyphenanthrene this difference was statistically significant < 0.05. Grilled marshmallows constitute a source of exposure to PAHs, especially in the group of children and adolescents. Even consumption of small or moderate amounts of grilled marshmallows resulted in a significant increase in concentrations of PAH metabolites in the urine compared to the level of these compounds before the intake.
棉花糖是一种深受世界各地儿童和青少年喜爱的糖果。烧烤以及食用烤棉花糖，尤其是儿童食用烤棉花糖，在许多国家已变得流行起来。烤棉花糖可能含有致癌的多环芳烃（PAHs）。已测定了志愿者食用烤棉花糖后尿液中羟基多环芳烃（OH-PAHs）的浓度，作为接触多环芳烃的生物标志物。共有24名参与者食用了在类似条件下烤制的棉花糖。在食用烤棉花糖前后收集尿液样本。通过酶水解和固相萃取（SPE）分离后，使用高效液相色谱-荧光检测（HPLC-FLD）技术测定尿液样本中1-羟基芘和9-羟基菲的浓度。1-羟基芘的平均浓度为0.21±0.16μg/g肌酐，9-羟基菲的平均浓度为2.78±2.55μg/g肌酐。与食用白色烤棉花糖相比，食用彩色烤棉花糖的志愿者尿液中OH-PAHs的浓度更高。就9-羟基菲而言，这种差异具有统计学意义（P<0.05）。烤棉花糖是多环芳烃的一个暴露源，尤其是在儿童和青少年群体中。与摄入前这些化合物的水平相比，即使食用少量或适量的烤棉花糖也会导致尿液中多环芳烃代谢物的浓度显著增加。
