Seibert Janaína Brandão, Amparo Tatiane Roquete, Sousa Lucas Resende Dutra, Rodrigues Ivanildes Vasconcelos, Petit Alicia, Pervier Pauline, Azevedo Mariana Costa, Sales Junior Policarpo Ademar, Murta Silvane Maria Fonseca, Carneiro Cláudia Martins, Teixeira Luiz Fernando de Medeiros, de Souza Gustavo Henrique Bianco, Vieira Paula Melo de Abreu, Santos Orlando David Henrique Dos
Department of Entomology and Acarology, "Luiz de Queiroz" College of Agriculture, Federal University of São Paulo, Piracicaba 13418-260, Brazil.
Department of Pharmacy, Federal University of Ouro Preto, Ouro Preto 35402-163, Brazil.
Pharmaceutics. 2025 Aug 29;17(9):1130. doi: 10.3390/pharmaceutics17091130.
The study of biological activity of plants and their metabolites is an important approach for the discovery of new active material. However, little is known of the properties of the genus. In addition to natural products, nanotechnology demonstrates considerable potential in pharmacotherapy. The utilization of nanoemulsions holds considerable promise in enhancing the efficacy of drugs, reducing dose, and therefore, lowering of toxic effects. In this context, antimicrobial and trypanocidal activities were evaluated to the free and encapsulated essential oil from in oil-in-water (o/w) nanoemulsion. This oil is composed mainly of -pinocarvyl acetate (~80.0%). The nanoemulsions were prepared by phase inversion method and showed mean particle size of 58 nm, polydispercity index of 0.09, pH 7.8, zeta potential of -21.9 mV, electrical conductivity of 0.38 mS/cm, and good stability. The essential oil was active against all five Gram-positive bacteria tested, and the formulation enhanced this ability. The cytotoxicity effect on L929 cells was also reduced after encapsulation of this oil in o/w nanoemulsion. In addition, the oil and the nanoemulsion were able to inhibit the growth of . Thus, the development of a nanoemulsion loaded with essential oil is an easy and low-cost way to obtain and deliver the -pinocarvyl acetate compound as well as allow its use in the treatment of diseases caused mainly by the genus and .
植物及其代谢产物的生物活性研究是发现新活性物质的重要途径。然而，对该属的特性了解甚少。除了天然产物外，纳米技术在药物治疗中也显示出巨大潜力。纳米乳剂的应用在提高药物疗效、降低剂量以及因此降低毒性作用方面具有很大的前景。在此背景下，对水包油（o/w）纳米乳剂中游离和包封的精油的抗菌和杀锥虫活性进行了评估。这种油主要由乙酸松油烯酯（约80.0%）组成。纳米乳剂通过相转变法制备，平均粒径为58nm，多分散指数为0.09，pH值为7.8，ζ电位为-21.9mV，电导率为0.38mS/cm，具有良好的稳定性。该精油对所测试的所有五种革兰氏阳性菌均有活性，且制剂增强了这种能力。将该油包封在o/w纳米乳剂中后，对L929细胞的细胞毒性作用也降低了。此外，该油和纳米乳剂能够抑制……的生长。因此，开发负载精油的纳米乳剂是一种简便且低成本的方法，可用于获取和递送乙酸松油烯酯化合物，并使其用于治疗主要由该属和……引起的疾病。
