Lu Yanfeng, Zhang Lei, Zhu Rui, Duan Xiujun, Sun Guobo, Jiang Yuying
School of Animal Science and Technology, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China.
Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Engineering Technology Research Center for Modern Animal Science and Novel Veterinary Pharmaceutic Development, Taizhou 225300, China.
Vet Sci. 2025 Aug 25;12(9):808. doi: 10.3390/vetsci12090808.
This study was conducted to investigate the effects of a Chinese herbal medicine compound microecological agent (C-MEA) on the egg production performance, ovarian follicle development, ovary transcriptome, and cecal microbiota of caged laying ducks. A total of 108 black Muscovy ducks (150 days old) were randomly divided into three groups for 30 days in a formal feeding trial. Compared with the control basic diet (Group C) and 16 g/kg C-MEA dosage (Group B), the 8 g/kg C-MEA dosage (Group A) increased egg production (average laying rate 69.35%) and follicle development (57 Fs, 67 LYFs, 11~13 SYFs) mass ( < 0.05). According to RNA-Seq, the ovaries' transcriptome among different dietary groups enriched six key pathways, including neuroactive ligand-receptor interaction, the PPAR signaling pathway, ECM-receptor interaction, focal adhesion, the adherens junction, and the FoxO signaling pathway, as well as 46 candidate key genes. According to 16S-Seq, the microbial diversity was significantly increased in Group A, and the genus abundances of and were significantly changed among different dietary groups ( < 0.05). Supplementation with C-MEA may optimize the cecal microflora and the interactions between the intestinal microflora and the host. The results from combining RNA-Seq and 16S-Seq demonstrated that the relationship between and the hub gene cluster (, , , , , , and ) is the most prominent. In conclusion, the egg performance of caged laying ducks can be modulated through the microbiota-gut-ovary axis. Our findings provide new insights for improving gut health and reproductive performance of caged laying ducks.
本研究旨在探讨一种中药复方微生态制剂（C-MEA）对笼养蛋鸭产蛋性能、卵巢卵泡发育、卵巢转录组和盲肠微生物群的影响。在正式饲养试验中，将108只150日龄的黑番鸭随机分为三组，为期30天。与对照基础日粮组（C组）和16 g/kg C-MEA剂量组（B组）相比，8 g/kg C-MEA剂量组（A组）的产蛋量（平均产蛋率69.35%）和卵泡发育（57个Fs、67个LYFs、11~13个SYFs）质量增加（P<0.05）。根据RNA测序，不同日粮组卵巢转录组富集了六个关键通路，包括神经活性配体-受体相互作用、PPAR信号通路、ECM-受体相互作用、粘着斑、紧密连接和FoxO信号通路，以及46个候选关键基因。根据16S测序，A组微生物多样性显著增加，不同日粮组的[具体菌属1]和[具体菌属2]菌属丰度发生显著变化（P<0.05）。添加C-MEA可能优化盲肠微生物群以及肠道微生物群与宿主之间的相互作用。RNA测序和16S测序相结合的结果表明，[具体菌属1]与核心基因簇（[具体基因1]、[具体基因2]、[具体基因3]、[具体基因4]、[具体基因5]、[具体基因6]和[具体基因7]）之间的关系最为突出。总之，笼养蛋鸭的产蛋性能可通过微生物群-肠道-卵巢轴进行调节。我们的研究结果为改善笼养蛋鸭的肠道健康和繁殖性能提供了新的见解。
