Lugo Tomas, Myers Stephanie, Nguyen Thu Annelise
School of Veterinary Medicine, Texas Tech University, Amarillo, TX 79106, USA.
Vet Sci. 2025 Sep 18;12(9):905. doi: 10.3390/vetsci12090905.
In vitro models have revolutionized our understanding of biological pathways and mechanisms, offering a viable alternative to direct patient testing. However, there is a significant lack of models for different animals, particularly equine models. This study presents a novel primary cell culture extracted from a 3-year-old horse diagnosed with multisystemic eosinophilic epitheliotropic disease. Tissue samples were collected from lymph nodes at various locations. Growth curves of extracted primary cells were analyzed and the optimal conditions were assessed. Biomarkers, such as CD31, ZO-1, CD79, Beta-catenin, E-cadherin, and LYE-1, were detected using an immunofluorescence assay, indicating that these primary cells are of endothelial origin. Initial whole-genome sequencing was performed to confirm the species' origin and to identify the number of common variations in comparison with the NIH EquCab3.0 reference genome. For the first time, the establishment of primary equine cells from lymph nodes is reported, and these can be used as an in vitro model for testing drug responses, molecular pathways, and environmental effects.
体外模型彻底改变了我们对生物途径和机制的理解，为直接在患者身上进行测试提供了一种可行的替代方案。然而，不同动物的模型严重匮乏，尤其是马的模型。本研究展示了一种从一匹被诊断患有多系统嗜酸性上皮嗜性疾病的3岁马身上提取的新型原代细胞培养物。从不同位置的淋巴结收集组织样本。分析提取的原代细胞的生长曲线并评估最佳条件。使用免疫荧光测定法检测生物标志物，如CD31、ZO-1、CD79、β-连环蛋白、E-钙黏蛋白和LYE-1，表明这些原代细胞来源于内皮细胞。进行了初步的全基因组测序，以确认物种来源，并与NIH EquCab3.0参考基因组比较，确定常见变异的数量。首次报道了从淋巴结建立马原代细胞，这些细胞可作为测试药物反应、分子途径和环境影响的体外模型。
