Rahimi Zahra, Mirhoseini Monirsadat, Eslamian Ghazaleh, Ghorbani Arman, Zand Hamid, Farahani Safoura Vasheghani
Department of Cellular and Molecular Nutrition, Faculty of Nutrition and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Cellular and Molecular Nutrition, Faculty of Nutrition and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, No 7, Hafezi St., Farahzadi Blvd, P.O.Box: 19395-4741, Tehran, 1981619573, Iran.
Genes Nutr. 2025 Sep 26;20(1):16. doi: 10.1186/s12263-025-00776-7.
Oxidative stress plays a pivotal role in cellular aging, and dietary antioxidants may modulate this process through regulation of the aging-related Klotho gene. This cross-sectional study investigated the association between dietary total antioxidant capacity (DTAC) and Klotho gene expression in 50 healthy Iranian adults (aged 18-40 years).
Using validated food frequency questionnaires, we calculated DTAC (mmol Trolox equivalents/day) and assessed Klotho mRNA levels in peripheral blood mononuclear cells (PBMCs) via RT-PCR (ΔCt method). Linear regression models evaluated these associations, with Model 1 showing crude associations and Model 2 adjusting for age, sex, BMI, waist circumference, physical activity, and fiber intake.
Unadjusted analysis revealed DTAC significantly predicted Klotho expression (β = -0.662, p < 0.001, R² = 0.438), with higher antioxidant intake associated with lower ΔCt values (indicating greater Klotho expression). This association remained significant after full adjustment (β = -0.599, p = 0.002, Adjusted R² = 0.381). Notably, among individual antioxidants, only zinc maintained an independent inverse relationship with ΔCt Klotho in the adjusted model (β = -0.439, p = 0.016), while other antioxidants lost significance.
These findings demonstrate that higher dietary antioxidant intake, particularly zinc, is significantly associated with increased Klotho gene expression, suggesting a potential mechanism by which antioxidant-rich diets may influence aging processes. This study provides novel evidence linking DTAC to Klotho expression in humans, supporting further investigation into dietary strategies for modulating aging-related pathways.
氧化应激在细胞衰老中起关键作用，膳食抗氧化剂可能通过调节与衰老相关的Klotho基因来调控这一过程。这项横断面研究调查了50名健康伊朗成年人（年龄在18 - 40岁之间）的膳食总抗氧化能力（DTAC）与Klotho基因表达之间的关联。
使用经过验证的食物频率问卷，我们计算了DTAC（毫摩尔Trolox当量/天），并通过逆转录聚合酶链反应（ΔCt法）评估外周血单个核细胞（PBMCs）中的Klotho mRNA水平。线性回归模型评估了这些关联，模型1显示了粗略关联，模型2对年龄、性别、体重指数、腰围、身体活动和纤维摄入量进行了调整。
未调整分析显示DTAC显著预测了Klotho表达（β = -0.662，p < 0.001，R² = 0.438），抗氧化剂摄入量越高，ΔCt值越低（表明Klotho表达越高）。在完全调整后，这种关联仍然显著（β = -0.599，p = 0.002，调整后R² = 0.381）。值得注意的是，在个体抗氧化剂中，在调整模型中只有锌与Klotho的ΔCt保持独立的负相关（β = -
