M带：荧光抗体染色研究
The M band. Studies with fluorescent antibody staining.
作者信息
Kundrat E, Pepe F A
出版信息
J Cell Biol. 1971 Feb;48(2):340-7. doi: 10.1083/jcb.48.2.340.
Abstract
The M band can be extracted from fibrils suspended in 5 mM Tris buffer, pH 8.0, for 15 min. The M band is completely removed only from fibrils of sarcomere lengths greater than 2.1 micro. Extraction does not alter the fluorescent antimyosin staining pattern of the A band, thus providing strong evidence that no alteration of the structural integrity of the thick filament has occurred. Fluorescent antibody staining of the M band of unextracted fibrils can be prevented specifically by absorbing the fluorescent antibody with extracted M band material prior to staining. This verifies the specificity of the extraction procedure.
摘要
M带可从悬浮于pH 8.0的5 mM Tris缓冲液中的肌原纤维中提取15分钟。仅从肌节长度大于2.1微米的肌原纤维中可完全去除M带。提取过程不会改变A带的荧光抗肌球蛋白染色模式，从而有力证明粗肌丝的结构完整性未发生改变。在染色前用提取的M带材料吸收荧光抗体，可特异性地阻止未提取肌原纤维M带的荧光抗体染色。这证实了提取过程的特异性。
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本文引用的文献
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"M-substance", a new protein constituting the M-line of myofibrils.“M物质”，一种构成肌原纤维M线的新蛋白质。
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