Nakane P K, Nichoalds G E, Oxender D L
Science. 1968 Jul 12;161(3837):182-3. doi: 10.1126/science.161.3837.182.
Rabbit antibody against lecine-binding protein isolated from Escherichia coli K-12 has been prepared. This antibody has been used in conjunction with enzyme-labeled antibody to allow an immunocytochemical localizati of leucine-binding protein in the Escherichil coli cell. This protein appears to be present only in the envelope and not in the cytoplasm.
已制备出针对从大肠杆菌K-12中分离的亮氨酸结合蛋白的兔抗体。该抗体已与酶标抗体联合使用,以便在大肠杆菌细胞中对亮氨酸结合蛋白进行免疫细胞化学定位。该蛋白似乎仅存在于包膜中,而不存在于细胞质中。