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来自大肠杆菌的亮氨酸结合蛋白的细胞定位

Cellular localization of leucine-binding protein from Escherichia coli.

作者信息

Nakane P K, Nichoalds G E, Oxender D L

出版信息

Science. 1968 Jul 12;161(3837):182-3. doi: 10.1126/science.161.3837.182.

DOI:10.1126/science.161.3837.182
PMID:4968602
Abstract

Rabbit antibody against lecine-binding protein isolated from Escherichia coli K-12 has been prepared. This antibody has been used in conjunction with enzyme-labeled antibody to allow an immunocytochemical localizati of leucine-binding protein in the Escherichil coli cell. This protein appears to be present only in the envelope and not in the cytoplasm.

摘要

已制备出针对从大肠杆菌K-12中分离的亮氨酸结合蛋白的兔抗体。该抗体已与酶标抗体联合使用，以便在大肠杆菌细胞中对亮氨酸结合蛋白进行免疫细胞化学定位。该蛋白似乎仅存在于包膜中，而不存在于细胞质中。

相似文献

1
Cellular localization of leucine-binding protein from Escherichia coli.来自大肠杆菌的亮氨酸结合蛋白的细胞定位
Science. 1968 Jul 12;161(3837):182-3. doi: 10.1126/science.161.3837.182.
2
Identification of a precursor pool of ribosome protein in Escherichia coli.大肠杆菌中核糖体蛋白前体库的鉴定。
J Bacteriol. 1968 Apr;95(4):1355-67. doi: 10.1128/jb.95.4.1355-1367.1968.
3
Reversible envelope effects during and after killing of Escherichia coli w by a highly-purified rabbit polymorpho-nuclear leukocyte fraction.用高度纯化的兔多形核白细胞组分杀死大肠杆菌w期间及之后的可逆包膜效应
Biochim Biophys Acta. 1976 Jun 4;436(1):154-69. doi: 10.1016/0005-2736(76)90227-3.
4
[Determination of leucine-binding protein content of Escherichia coli cells].
Biokhimiia. 1978 Jan;43(1):17-22.
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Derepressed leucine transport activity in Escherichia coli.大肠杆菌中去阻遏的亮氨酸转运活性。
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Reversible association as a possible regulatory mechanism for controlling the activity of the non-specific leucine-binding protein from Escherichia coli.
Adv Enzyme Regul. 1976;14:269-78. doi: 10.1016/0065-2571(76)90017-0.
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Turnover of protein in Escherichia coli starving for nitrogen.氮饥饿状态下大肠杆菌中蛋白质的周转
Biochim Biophys Acta. 1966 Apr 18;119(1):171-82. doi: 10.1016/0005-2787(66)90048-7.
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Transport of sugars and amino acids in bacteria. XII. Substrate specificities of the branched chain amino acid-binding proteins of Escherichia coli.细菌中糖和氨基酸的转运。十二、大肠杆菌支链氨基酸结合蛋白的底物特异性
J Biochem. 1974 Dec;76(6):1165-73. doi: 10.1093/oxfordjournals.jbchem.a130669.
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[Species specificity in leucyl-ribonucleic acid formation from Escherichia coli and from rabbit reticulocytes].[从大肠杆菌和兔网织红细胞形成亮氨酰核糖核酸中的物种特异性]
Arch Int Physiol Biochim. 1968 Dec;76(5):943-4.
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In vivo footprinting analysis of Lrp binding to the ilvIH promoter region of Escherichia coli.大肠杆菌ilvIH启动子区域Lrp结合的体内足迹分析。
J Bacteriol. 1994 Sep;176(17):5197-201. doi: 10.1128/jb.176.17.5197-5201.1994.

引用本文的文献

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Influence of Turgor Pressure Manipulation on Plasmalemma Transport of HCO(3) and OH in Chara corallina.膨压操控对珊瑚藻质膜中 HCO(3)和 OH 运输的影响。
Plant Physiol. 1981 Sep;68(3):553-9. doi: 10.1104/pp.68.3.553.
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Loss of membrane transport ability in leaf cells and release of protein as a result of osmotic shock.由于渗透冲击导致叶细胞的膜转运能力丧失和蛋白质释放。
Plant Physiol. 1973 Apr;51(4):620-5. doi: 10.1104/pp.51.4.620.
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J Bacteriol. 1971 Sep;107(3):878-88. doi: 10.1128/jb.107.3.878-888.1971.
4
Structure and function of the cell envelope of gram-negative bacteria.革兰氏阴性菌细胞包膜的结构与功能
Bacteriol Rev. 1974 Mar;38(1):87-110. doi: 10.1128/br.38.1.87-110.1974.
5
Localization of phosphoglucose isomerase in Escherichia coli and its relation to the induction of the hexose phosphate transport system.磷酸葡萄糖异构酶在大肠杆菌中的定位及其与磷酸己糖转运系统诱导的关系。
J Bacteriol. 1972 Dec;112(3):1201-5. doi: 10.1128/jb.112.3.1201-1205.1972.
6
Solubilization and partial purification of amino acid-specific components of the D-lactate dehydrogenase-coupled amino acid-transport systems (E. coli-cell membranes-sephadex-detergent-solubilized-vesicles).D-乳酸脱氢酶偶联氨基酸转运系统（大肠杆菌细胞膜-葡聚糖凝胶-去污剂增溶囊泡）中氨基酸特异性组分的增溶及部分纯化
Proc Natl Acad Sci U S A. 1972 Feb;69(2):358-62. doi: 10.1073/pnas.69.2.358.
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Cytochemical localization of certain phosphatases in Escherichia coli.大肠杆菌中某些磷酸酶的细胞化学定位
J Bacteriol. 1970 Oct;104(1):529-42. doi: 10.1128/jb.104.1.529-542.1970.
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Use of horseradish peroxidase-labeled antibody for light and electron microscope localization of reovirus antigen.辣根过氧化物酶标记抗体用于呼肠孤病毒抗原的光镜和电镜定位
Appl Microbiol. 1971 Mar;21(3):534-8. doi: 10.1128/am.21.3.534-538.1971.
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J Bacteriol. 1978 Sep;135(3):1080-90. doi: 10.1128/jb.135.3.1080-1090.1978.