Nonomura I, Yoder H W
Avian Dis. 1977 Jul-Sep;21(3):370-81.
Recent isolates of Mycoplasma gallisepticum and Mycoplasma synoviae were readily typed by the agar-gel precipitin test with antigens prepared by freezing and thawing, sonic vibration, or sodium dodecyl sulfate. Specific antisera prepared in rabbits or in foot-pad-inoculated chickens were adequate for culture typing. Relatively few sera from chickens and turkeys in naturally infected flocks reacted positively. The precipitin reaction was highly specific, however.
利用通过冻融、超声振动或十二烷基硫酸钠制备的抗原,通过琼脂凝胶沉淀试验可轻松对近期分离出的鸡毒支原体和滑液支原体进行分型。用兔或经足垫接种的鸡制备的特异性抗血清足以用于培养物分型。自然感染鸡群中鸡和火鸡的血清相对较少呈阳性反应。然而,沉淀反应具有高度特异性。