Identification of avian mycoplasma isolates by the agar-gel precipitin test.

Recent isolates of Mycoplasma gallisepticum and Mycoplasma synoviae were readily typed by the agar-gel precipitin test with antigens prepared by freezing and thawing, sonic vibration, or sodium dodecyl sulfate. Specific antisera prepared in rabbits or in foot-pad-inoculated chickens were adequate for culture typing. Relatively few sera from chickens and turkeys in naturally infected flocks reacted positively. The precipitin reaction was highly specific, however.