Cummins D R, Reynolds D L
Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Ames, Iowa 50010.
Avian Dis. 1990 Apr-Jun;34(2):321-8.
A dot-immunobinding assay, amplified with avidin and biotin (DAB assay), was used to detect serum antibodies to Mycoplasma iowae in immunized turkeys. The DAB assay was used to test serum samples from 122 commercial market turkey flocks obtained from four Iowa processing plants. The samples were pooled and tested for the presence of antibodies to four species of Mycoplasma spp. considered to be important pathogens for turkeys: M. gallisepticum (MG), M. iowae (MI), M. meleagridis (MM), and M. synoviae (MS). The occurrence of antibodies against these mycoplasmas, as determined by the DAB assay, were 5.7% for MG, 18.0% for MI, 77.9% for MM, and 9.8% for MS.
采用一种用抗生物素蛋白和生物素放大的斑点免疫结合试验（DAB试验）来检测免疫火鸡血清中抗鸡败血支原体的抗体。DAB试验用于检测从爱荷华州四个加工厂采集的122个商业市场火鸡群的血清样本。将样本合并，检测针对四种被认为是火鸡重要病原体的支原体属物种的抗体：鸡毒支原体（MG）、伊氏支原体（MI）、火鸡支原体（MM）和滑液支原体（MS）。通过DAB试验确定，针对这些支原体的抗体出现率分别为：MG为5.7%，MI为18.0%，MM为77.9%，MS为9.8%。
