Reid V, Glaser M, Kennett R, Singer S J
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1184-7. doi: 10.1073/pnas.68.6.1184.
Two homogeneous univalent hapten-protein conjugates, prepared by the covalent attachment of a single 2,4-dinitrophenyl (DNP-) or 2,4,6-trinitrophenyl (TNP-) side chain to the cysteine-SH in the active site of the enzyme papain, have been found to exhibit large Cotton effects in the wavelength region of the absorption bands of the DNP or TNP groups. This indicates that the DNP or TNP groups are noncovalently bound to some asymmetric region(s) of the papain molecule. These homogeneous papain conjugates are antigens that can elicit anti-DNP or anti-TNP antibody production in mice or rabbits. It is suggested that the noncovalent binding of a hapten to the surface of its carrier protein may profoundly affect the characteristics of the anti-hapten antibodies that are elicited. It has also been observed that the specific binding of these papain conjugates, and of the simple haptens DNP-lysine or TNP-lysine, to anti-hapten antibodies produces characteristic extrinsic Cotton effects.
通过将单个2,4 - 二硝基苯基(DNP-)或2,4,6 - 三硝基苯基(TNP-)侧链共价连接到木瓜蛋白酶活性位点的半胱氨酸-SH上制备的两种均一的单价半抗原-蛋白质缀合物,已发现在DNP或TNP基团吸收带的波长区域表现出大的科顿效应。这表明DNP或TNP基团与木瓜蛋白酶分子的一些不对称区域非共价结合。这些均一的木瓜蛋白酶缀合物是抗原,可在小鼠或兔子中引发抗DNP或抗TNP抗体的产生。有人提出,半抗原与其载体蛋白表面的非共价结合可能会深刻影响所引发的抗半抗原抗体的特性。还观察到,这些木瓜蛋白酶缀合物以及简单的半抗原DNP-赖氨酸或TNP-赖氨酸与抗半抗原抗体的特异性结合会产生特征性的外在科顿效应。