Further evidence for the involvement of microtubules in the intra-axonal movement of noradrenaline storage granules.

1. Constricted cat hypogastric nerve/inferior mesenteric ganglion preparations maintained in vitro for 48 hr have been used to study the effects of different concentrations of colchicine on axonal microtubules and on the proximo-distal movement of catecholamine containing dense-cored vesicles in non-myelinated axons.2. In low concentrations (0.03-0.3 mug/ml.), colchicine had no effect on the number of microtubules per axon and did not diminish the amount of noradrenaline accumulating proximal to the constriction.3. Higher concentrations of colchicine (1.0-10 mug/ml.) produced a dramatic reduction in the number of microtubules per axon. This was associated with marked reductions in the number of dense-cored vesicles and the amount of noradrenaline accumulating above the constriction.4. There was some morphological evidence for a structural relationship between dense-cored vesicles and microtubules.5. These results further support the view that axonal microtubules are involved in the relatively fast proximo-distal transport of noradrenaline containing dense-cored vesicles in non-myelinated sympathetic axons.